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Introduction: We earlier showed in an animal model that steatosis, in the absence of fibrosis or inflammation, induces a significant rise in portal pressure. The relevance of these findings for human pathology is unknown till date., Aims: To study portal pressure in nonalcoholic fatty liver disease patients and to identify factors possibly related to steatosis-induced changes in liver haemodynamics., Materials and Methods: Patients presenting with a problem of overweight and with noninvasive signs of liver involvement were proposed for transjugular liver biopsy. The biopsy was scored according to the Nonalcoholic Steatohepatitis Clinical Research Network scoring system., Results: Fifty consecutive patients were studied. Mean age was 47.9 ± 13.6 years; 31 (62%) of them were female. Hepatic venous pressure gradient (HVPG) was normal in 27 patients (54%), borderline (5 mmHg) in nine (18%) and elevated in 14 patients (28%). For further analysis those with a HVPG of 5 mmHg were considered normal (group 1). HVPG was 8.8 ± 2.6 mmHg in those with an elevated HVPG (group 2) versus 3.4 ± 1.2 mmHg in group 1 (P < 0.0001). In both the groups, only one patient had cirrhosis; 26 of 36 (group 1) and nine of 14 patients (group 2) had fibrosis score 0. Fibrosis score was not significantly different (P = 0.530). Perisinusoidal fibrosis score was not significantly different (P = 0.186). Steatosis was the only histological feature that significantly differed between the groups (P = 0.016). The degree of steatosis (P = 0.010) was the only independent predictor of the presence of portal hypertension., Conclusion: Human nonalcoholic fatty liver disease can, even in the absence of significant fibrosis, induce portal hypertension, correlated with the severity of the steatosis.

Population: One hundred and thirteen unrelated Flemish men from Belgium.

Aims: Intratumoural micro-vessel density (IMD) has recently been shown to be a valuable prognostic tool in many tumours. Yet, IMD does not take into account the spatial arrangement of the vessels, therefore only partly reflecting the angiogenic situation. In order to describe contextual vascular relationships more accurately, we have used fractal and syntactic structure analysis (SSA) based on computerised image processing to quantify micro-vascular hot spots., Materials and Methods: The parametric performance in prediction of patients' outcome was evaluated by univariate analysis and compared with manually obtained IMDs, whereas an automated K-nearest-neighbour (KNN) classifier searched most discriminative parametric combinations. The method is based on analysis of vascular 'hot-spots' of paraffin-embedded tissue sections of invasive cervical carcinoma, colorectal carcinoma and malignant mesothelioma., Results: For all three cancers, prediction of prognosis based on SSA yielded in general much higher recognition scores compared with IMD or fractal dimension. Survival of cervical carcinoma was mostly correlated with clinical data, with the vascular permeation being the only parameter with independent value. Prognosis of colorectal carcinoma is best described by SSA, completed with IMD, indicating an inverse correlation of survival time with a more irregular pattern and a slight increase in vessel number. For mesothelioma, we found a strong correlation with SSA and patients' outcome, with two SSA-parameters having independent prognostic value., Conclusions: The more accurate angiogenic description obtained with SSA may be useful for further exploitation as a prognosticator in a general diagnostic pathology service.

Objective: The frequent association of a monkey oncogenic virus, simian virus 40 (SV40), with pleural mesothelioma and the proposed transmission of the virus from contaminated polio vaccines to humans has received considerable scientific and public attention. We sought to determine whether SV40 would indeed be present in mesothelioma patients from Belgium, as claimed in former studies, and to characterize the viral genome in respective specimens., Methods: DNA was extracted from frozen tissue from 12 patients diagnosed with pleural mesothelioma in Belgium. Five different extraction methods were compared and primer pairs targeting four informative regions of the SV40 genome were used to amplify viral products by the polymerase chain reaction (PCR). One of the pairs additionally allows amplification of human polyoma viruses. Southern blotting with an oligonucleotide probe directed at the expected SV40 sequences was also applied., Results: One of the 12 samples contained amplifiable JC virus DNA. In contrast, none of the samples (0/12, 95% CI 0% to 26.5%) was positive for SV40 DNA sequences. The Southern blot analysis confirmed the absence of trace amounts of SV40 PCR product. We also clearly demonstrate that an otherwise specific probe against SV40 could easily hybridize in quite a non-specific manner under recommended conditions., Conclusions: These results provide strong evidence for a lack of association of SV40 with most pleural mesotheliomas in the Belgian population. We recommend a re-examination of other positive case series and avoidance of questionable hybridization practices in future studies.

Worldwide there is a strong relation between the presence of human papillomavirus (HPV) and the development of cervical cancer. This study investigated the prevalence and genotype of HPV in women with normal smears, women with premalignant lesions and women with cervical cancer in Antwerp, Belgium. Type-specific polymerase chain reaction (PCR) for HPV types 16 and 18 and general primer PCR (GP5+/6+) was performed on DNA extracted from paraffin-embedded tissue from women with lesions or fresh material from controls. HPV was detected in 11% of controls, 61% of women with atypia, 77% of women with CIN lesions and 88% of women with cervical carcinoma (chi2 trend, 273, p<0.001). The odds ratio for high-risk HPV types was 9.3 for atypia (95%CI. 4.3-19.8), 33.6 for CIN lesions (95%CI, 19.3-58.6) and 78.8 for cervical cancer (95%CI, 39.2-158.3). In total, 19 different HPV genotypes were detected, including five low risk HPV types. Seven of the 14 high-risk HPV types were detected in cervical cancer patients. Based on our study it is suggested that a prophylactic vaccine based on a cocktail of a limited number of high-risk HPV types should be considered in order to protect most women from developing cervical cancer.

Two imported cases of Penicillium marneffei infection in Belgium are reported. Both patients are Thai women co-infected with HIV. P. marneffei infection should be suspected in immunocompromised patients originating or travelling from South-East Asia with unexplained fever (> 38 degrees C), weight loss, a generalised lymphadenopathy, hepatomegaly, splenomegaly, skin lesions, cough and anaemia. Diagnosis is made by culture and/or histopathological examination. Mild to moderate infections are treated with itraconazole 400 mg/day as first choice. Amphotericin B parenteral therapy may be required for seriously ill patients. Maintenance therapy with itraconazole 200 mg/day is necessary to prevent relapses.

The prevalence of hairy leukoplakia was determined among 176 symptomatic HIV seropositive patients seen at the outpatient department of the Institute of Tropical Medicine in Antwerp, Belgium. Moreover, systematic tongue biopsies were performed during postmortem examination of 21 patients with AIDS, 100 HIV seronegative immunocompromised patients with haematologic or other malignancies and 100 HIV seronegative non-immunocompromised patients who died at the University Hospital Antwerp. Hairy leukoplakia was observed in 52 (29.5%) of the outpatients, but only in one (5%) of the AIDS patients in the postmortem study (P = 0.03). An explanation for this difference may be that significantly more AIDS patients who died had received either acyclovir or ganciclovir during the 3 months prior to the postmortem examination than the HIV seropositive outpatients during the 3 months prior to examination. Hairy leukoplakia occurred more often in Caucasian homosexual men with HIV infection (38%) than among heterosexual Africans with HIV infection (17%) (P = 0.06). Hairy leukoplakia was observed in none of the HIV seronegative patients.