1. Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population.
- Author
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Xuan-Yi Wang, Ansaruzzaman, M, Vaz, Raul, Mondlane, Catarina, Lucas, Marcelino ES, von Seidlein, Lorenz, Deen, Jacqueline L, Ampuero, Sonia, Puri, Mahesh, Park, Taesung, Nair, GB, Clemens, John D, Chaignat, Claire-Lise, Rajerison, Minoarisoa, Nato, Farida, and Fournier, Jean-Michel
- Subjects
CHOLERA diagnosis ,VIBRIO infections ,GRAM-negative bacterial diseases - Abstract
Background: Early detection of cholera outbreaks is crucial for the implementation of the most appropriate control strategies. Methods: The performance of an immunochromatographic dipstick test (Institute Pasteur, Paris, France) specific for Vibrio cholerae O1 was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season (January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture. Results: The overall sensitivity and specificity of the rapid test compared to culture were 95% (95% confidence interval [CI]: 91%-99%) and 89% (95% CI: 86%-93%), respectively. After stratification by type of sample (rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%. Conclusion: This one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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