1. Comparative analysis of Mycobacterium tuberculosis Beijing strains isolated in three remote areas of Japan.
- Author
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Yokoyama, Eiji, Hachisu, Yushi, Iwamoto, Tomotada, Nakanishi, Noriko, Arikawa, Kentaro, Wada, Takayuki, Seto, Junji, and Kishida, Kazunori
- Subjects
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MYCOBACTERIUM tuberculosis , *TANDEM repeats , *EPIDEMICS , *POPULATION genetics - Abstract
A quantitative and qualitative comparison was carried out of Mycobacterium tuberculosis Beijing strains isolated in three remote areas of Japan. A total of 452 strains from Chiba Prefecture, 75 from Yamagata Prefecture, and 315 from Kobe City were analyzed for 24 loci by variable number of tandem repeats typing (24 Beijing -VNTR). All strains were classified in six Beijing subgroups (B SUB ), B1 to B5 and T, based on a minimum spanning tree reconstructed using data of a standard set of 15 VNTR loci. No significant difference was found in the distribution of strains in the B SUB in the three areas, with one exception due to a B5 outbreak in Yamagata, indicating no significant quantitative difference in the B SUB in the three areas ( P < 0.01, Chi -square test). In addition, when strains in each B SUB isolated in the three areas were mixed and standardized index of association ( I A s ) and variance ( Φ PT ) values were calculated, no significant qualitative difference in the B SUB in the three areas was found. These results suggested that the B SUB diverged prior to the introduction of M. tuberculosis Beijing strains into Japan. Differences in the distribution of strains in each B SUB between Japan and continental Asian countries suggested there had been genetic drift in the continental Asian countries in which B4 had been dominant. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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