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We present a coupled macroscopic traffic and emission modelling system tailored to the Barcelona metropolitan area that allows estimating hourly road transport emissions at road link level. We use the developed system to perform an emission sensitivity analysis of typically high uncertainty emission features and assess their impact. We also explore the uncertainties of our system compared to a microscopic approach in a representative area of Barcelona. The developed macroscopic system shows a high sensitivity to spatially-resolved vehicle fleet composition inputs, meteorological effects on diesel engines (+ 19% in NO x) and non-exhaust sources (80% of total PM emissions). The comparison with the microscopic system shows that discrepancies grow as a function of the congestion level, up to + 65% in NO x. The resulting coupled system will be used in further steps of the research to evaluate the impact of traffic management strategies upon urban emissions and air quality levels in Barcelona. • The coupled system provides dynamic street-level emissions across 265k street segments. • Satisfactory overall behaviour despite some limitations in heavy traffic conditions. • Accounting for real public bus routes and non-exhaust PM sources has a strong impact. • The system will help evaluating the impact of mobility policies on air quality. [ABSTRACT FROM AUTHOR]