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A recent study conducted by researchers at Kasetsart University in Bangkok, Thailand, explored the effects of various factors on the knockdown and mortality of malaria-carrying mosquitoes. The study found that mosquito density, age, and nutritional status can impact the results of bioassay tests used for insecticide resistance monitoring and malaria transmission research. The researchers discovered that older mosquitoes were more susceptible to the insecticide transfluthrin, particularly Anopheles minimus. The study supports the World Health Organization's recommendation to use 3- to 5-day-old mosquitoes for these types of tests. [Extracted from the article]