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The Faculty of Medicine at Syiah Kuala University (USK) employees play a significant role in the continuity of the faculty's work. Even though working from home has been applied during the pandemic, in person meetings with health protocols are sometimes needed. Many employees have been confirmed positive for coronavirus infection. There are many reports of further effects on the cardiovascular organs of patients. The role of serum 25-hydroxyvitamin D as an immunomodulator has been proven, and the absence of comorbid factors such as hypertension, diabetes, obesity, and smoking may protect against reinfection. This study aimed to evaluate the physical health parameters, hs-Troponin I, 25-Hydroxyvitamin D serum, and comorbid factors of Faculty of Medicine USK employees with confirmed COVID-19. A cross-sectional study was conducted at two sites, the Faculty of Medicine USK and the dr. Zainoel Abidin Hospital Banda Aceh, to assess serum 25-hydroxyvitamin D levels, hs-Troponin I levels, and heart muscle fitness of positive participants. COVID-19 respondents with confirmatory PCR swab > 1 month, aged 20–45 years, mild symptoms, and a history of coronary heart disease or lower limb disability were not included in this study. A total of 30 USK Medical Faculty employees were included in this study. The research was carried out with permission from the Faculty of Medicine USK Health Research Ethics Committee. Blood samples for serum 25-hydroxyvitamin D and hs-troponin I were tested five days before the cardiac fitness test. Cardiac fitness checks carried out at the Cardiac Rehabilitation Unit of RSUDZA hospital were: 6-minute walk test (6MWT) and Heart Rate Variability. The mean value of 25-Hydroxyvitamin D serum was 18.4 ng/ml (insufficiency), and only two employees (0.06%) had 25-Hydroxyvitamin D serum levels within normal limits. There were 28 (93%) employees with hs-Troponin I values of 3.2 pg/ml, and two with abnormal values of 13.7 pg/ml and 30.6 pg/ml, respectively. There were four employees (13.3%) who had comorbid diabetes, hypertension, and obesity. Based on Heart Rate Variability results, 25 employees (83.3%) with heart signal problems require further monitoring. The results also revealed that 11 (36.7%) employees had limited 6-minute walk test (6MWT) ability. This study has not obtained satisfying results because the cardiovascular fitness variable still needs further analysis. Employees confirmed positive for COVID-19 had low 25-Hydroxyvitamin D serum levels and hs-Troponin I was within the normal range. Less than half of the respondents had limited 6-minute walk test (6MWT) ability, and the cardiac fitness scores still needed further analysis. [ABSTRACT FROM AUTHOR]