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Gut bacterial communities play a vital role in a host's digestion and fermentation of complex carbohydrates, absorption of nutrients, and energy harvest/storage. Dugongs are obligate seagrass grazers with an expanded hindgut and associated microbiome. Here, we characterised and compared the faecal bacterial communities of dugongs from genetically distinct populations along the east coast of Australia, between subtropical Moreton Bay and tropical Cleveland Bay. Amplicon sequencing of fresh dugong faecal samples (n=47) revealed Firmicutes (62%) dominating the faecal bacterial communities across all populations. Several bacterial genera (Bacteroides, Clostridium sensu stricto 1, Blautia and Polaribacter) were detected in samples from all locations, suggesting their importance in seagrass digestion. Principal coordinate analysis showed the three southern-most dugong populations having different faecal bacterial community compositions from northern populations. The relative abundances of the genera Clostridium sensu stricto 13 and dgA-11 gut group were higher, but Bacteroides was lower, in the southern dugong populations, compared to the northern populations, suggesting potential adaptive changes associated with location. This study contributes to our knowledge of the faecal bacterial communities of dugongs inhabiting Australian coastal waters. Future studies of diet selection in relation to seagrass availability throughout the dugong's range will help to advance our understanding of the roles that seagrass species may play in affecting the dugong's faecal bacterial community composition. [ABSTRACT FROM AUTHOR]

Cattle enterprises are one of the major livestock production systems globally and are forecasted to have stable growth in the next decade. To facilitate sustainable live weight production, optimal reproductive performance is essential. Microbial colonisation in the reproductive tract has been demonstrated as one of the factors contributing to bovine reproductive performance. Studies also implied that reproductive metagenomes are different at each stage of the estrous cycle. This study applied Oxford Nanopore Technologies' adaptive long-read sequencing to profile the bovine reproductive microbiome collected from tropical cattle in northern Queensland, Australia. The microbiome samples were collected from cattle of different sexes, reproductive status and locations to provide a comprehensive view of the bovine reproductive microbiome in northern Australian cattle. Ascomycota, Firmicutes and Proteobacteria were abundant phyla identified in the bovine reproductive metagenomes of Australian cattle regardless of sexes, reproductive status and location. The species level taxonomical investigation suggested that gastrointestinal metagenome and the surrounding environment were potentially the origins of the bovine reproductive metagenome. Functional profiles further affirmed this implication, revealing that the reproductive metagenomes of the prepubertal and postpartum animals were dominated by microorganisms that catabolise dietary polysaccharides as an energy substrate while that of the pregnant animals had the function of harvesting energy from aromatic compounds. Bovine reproductive metagenome investigations can be employed to trace the origins of abnormal metagenomes, which is beneficial for disease prevention and control. Additionally, our results demonstrated different reproductive metagenome diversities between cattle from two different locations. The variation in diversity within one location can serve as the indicator of abnormal reproductive metagenome, but between locations inferences cannot be made. We suggest establishing localised metagenomic indices that can be used to infer abnormal reproductive metagenomes which contribute to abortion or sub-fertility. [ABSTRACT FROM AUTHOR]

The utilization of forest harvest residues for renewable bioenergy production and bioproducts has increasingly become an integrated part of forestry that helps to meet the needs of climate change mitigation and a future carbon neutral economy. An essential element in the planning of any harvesting residue recovery operation is a reliable assessment of the quantity and quality of residue biomass and its composition over a harvest area. With the now widely adopted cut-to-length (CTL) at the stump harvesting system in Pinus radiata plantations in Australia, harvesting residues left on site are significantly larger in quantity and spatially more dispersed over a harvest area in comparison to the more traditional whole-tree harvesting. The conventional approach of assessing forest harvest residues through sample plots, transects, or small study blocks has provided site-specific estimates of residue biomass. However, these estimates cannot be readily extrapolated over the plantation landscape, which varies in silviculture, site, and stand conditions. To overcome this limitation, this study relied on harvester data analytics to obtain spatially explicit estimates of residue biomass using an example data set from harvested plantations of three stand types: unthinned (T0), thinned once (T1), and thinned twice (T2). Three methods of integrating biomass equations with harvester data were compared for residue biomass estimation: (1) applying individual tree biomass equations to harvested stems, (2) applying stand-level biomass equations to gridded harvester data, and (3) integrating estimates from the first approach with recorded and estimated waste volumes of harvested stems. The estimates of total residue biomass obtained using the three methods through harvester data analytics varied between 56.2 and 156.4 t/ha in green weight across the three stand types. These estimates were validated indirectly through ex situ sample plots and proved to be comparable to the quantities of residue biomass assessed using conventional sample plots, transects, or small blocks following CTL harvesting of rotation age P. radiata plantations elsewhere in Australia. Among the three methods, the third method made the most intensive use of the harvester data and provided the most realistic estimates of residue biomass. Spatial mapping of the estimated total and component residue biomass will assist the operational planning of residue recovery and site-specific nutrient management for the long-term sustainability of P. radiata plantations. [ABSTRACT FROM AUTHOR]

Offshore wind energy and wave energy are two exploitable sources of renewable energy available in the same marine environment. A co-located energy harvesting system of these two resources has the potential to play a leading role in the recent renewable energy mix mandate. A co-located system of mature offshore wind technology and a nascent wave energy technology can exploit the synergies of the two technologies, including the reduced cost, the easy operation and maintenance, and more importantly the potential of decreased power variability. This paper deals with a multi-site analysis of wind and wave power resources, and the estimation of exploitable energy through different wind turbines and wave energy converters (WECs). Results are presented for NSW Australia, but the devised strategy is generic and can be implemented in any region. Ten WECs, with different operating principles, are used for the estimation of the exploitable wave power, and the best performing converter is considered for the co-located wind-wave farm. A power fluctuation factor is introduced to quantify the reduction in power variability of the co-located wind-wave farm. Different percentage mixes of wind and wave energy are evaluated for the co-located wind-wave farm in the multi-site analysis. •A multi-site analysis of wind and wave for estimation of exploitable energy. •Performance evaluation of wind and wave energy conversion technologies. •Power variability representation with power fluctuation factor for a co-located farm. •Characterization of percentage share of wind and wave in a co-located farm. [ABSTRACT FROM AUTHOR]

Most herbivorous mammals have symbiotic microbes living in their gastrointestinal tracts that help with harvesting energy from recalcitrant plant fibre. The bulk of research into these microorganisms has focused on samples collected from faeces, representing the distal region of the gastrointestinal (GI) tract. However, the GI tract in herbivorous mammals is typically long and complex, containing different regions with distinct physico-chemical properties that can structure resident microbial communities. Little work has been done to document GI microbial communities of herbivorous animals at these sites. In this study, we use 16S rRNA gene sequencing to characterize the microbial biogeography along the GI tract in two species of wombats. Specifically, we survey the microbes along four major gut regions (stomach, small intestine, proximal colon, distal colon) in a single bare-nosed wombat (Vombatus ursinus) and a single southern hairynosed wombat (Lasiorhinus latifrons). Our preliminary results show that GI microbial communities of wombats are structured by GI region. For both wombat individuals, we observed a trend of increasing microbial diversity from stomach to distal colon. The microbial composition in the first proximal colon region was more similar between wombat species than the corresponding distal colon region in the same species. We found several microbial genera that were differentially abundant between the first proximal colon (putative site for primary plant fermentation) and distal colon regions (which resemble faecal samples). Surprisingly, only 10.6% (98) and 18.8% (206) of amplicon sequence variants (ASVs) were shared between the first proximal colon region and the distal colon region for the bare-nosed and southern hairy-nosed wombat, respectively. These results suggest that microbial communities in the first proximal colon region--the putative site of primary plant fermentation in wombats--are distinct from the distal colon, and that faecal samples may have limitations in capturing the diversity of these communities. While faeces are still a valuable and effective means of characterising the distal colon microbiota, future work seeking to better understand how GI microbiota impact the energy economy of wombats (and potentially other hindgut-fermenting mammals) may need to take gut biogeography into account. [ABSTRACT FROM AUTHOR]

Energy-efficient location tracking with battery-powered devices using energy harvesting necessitates duty-cycling of GPS to prolong the system lifetime. We propose an energy- and mobility-aware scheduling framework that adapts to real-world dynamics to achieve optimal long-term tracking performance. To forecast energy, the framework uses an exponentially weighted moving average filter to compute a virtual energy budget for the remainder of the forecast period. The virtual energy budget is then used as input for our proposed information-based GPS sampling approach, which estimates the current tracking error through dead-reckoning and schedules a new GPS sample when the error exceeds a given threshold. In order to improve the long-term tracking performance, the threshold is adapted based on the current energy and movement trends to balance the expected information gain from a new GPS sample with its energy cost. We evaluate our approach on empirical traces from wild flying foxes and compare it to strategies that sample GPS using fixed and adaptive duty cycles and by using dead-reckoning with a fixed threshold. Our analysis shows that the proposed information-based GPS sampling strategy reduces the mean tracking error compared to existing methods and approaches the performance of the optimal offline sampling strategy. [ABSTRACT FROM AUTHOR]

Piezoelectric energy harvesters (PEHs) can be used as an additional power supply for a Structural Health Monitoring (SHM) system. Its design can be optimised for the best performance, however, the optimal design depends on the input vibration and locations. In this work, we extend an optimisation framework from our previous studies to include the effect of the PEH's location, which uses the cantilevered PEH Kirchhoff–Love plate model discretised by IsoGeometric Analysis (IGA) and coupled with Particle Swarm Optimisation (PSO) algorithm to find the designs with maximum energy outputs for a large number of input acceleration histories, extracted from the recorded dynamic response data of a real cable-stayed bridge in Australia. Then, clustering and evaluation under 24-h excitation are performed to find several best PEHs for the entire bridge. By comparing the best PEH at all locations with a typical benchmark device, the substantial performance improvement brought by the optimisation framework of this work is verified. The comparison results show a significant energy harvesting enhancement of 1.6 times at the best locations, 2.4 times at A2 and A3, and 3.6 times at the edge girders, respectively. The results also reveal the variability of the best design at different locations of the same structure to maximise the energy harvesting of the entire bridge, and demonstrate the design rule that the fundamental frequency of the device can be tuned within a certain frequency range to improve the robustness of PEHs design. Additional studies are performed to explore the effect of location and traffic intensity on energy harvesting by analysing the optimal PEH design and location throughout the bridge structure. The results indicate that the key factors of maximising energy harvesting efficiency are related to the input excitation and the mode of vibration being excited. The position of maximum displacement in the vibration mode corresponds to the best location for energy harvesting. Also, the best device has a fundamental frequency close to the frequency of the corresponding vibration mode. In addition, the change of traffic intensity affects the amount of convertible mechanical energy and also directs the tuning of the fundamental frequency of the PEH to achieve the highest energy conversion. • A comprehensive optimisation design framework for PEHs on bridges is presented. • The best design and location of a PEH from an operating bridge is identified. • The relationship between the optimal design and the traffic intensity is determined. [ABSTRACT FROM AUTHOR]

Vibrational energy harvested from the bridge excitation due to the traffic flow or the wind load can be supplied to sensors in a Structural Health Monitoring (SHM) system and help prolong its service life, reduce chemical battery waste and enable its use in remote locations. A common approach to designing a Piezoelectric Energy Harvester (PEH) consists in tuning its fundamental frequency to some target value (e.g., the fundamental frequency of a bridge). However, such approach does not answer the question if the chosen target frequency is optimal, and does not take into account the possibility to have multiple design configurations with the same fundamental frequency. In this work, we approach the problem of a PEH design optimisation in a rigorous way, using a PEH model based on the Kirchhoff–Love plate theory and Isogeometric Analysis (IGA), coupled with the Modal Order Reduction (MOR) approach and Runge–Kutta time integration method. The model is further equipped with the Particle Swarm Optimisation (PSO) algorithm that allows finding geometry which maximises energy output from a given base acceleration signal. A comprehensive study is conducted to infer the impact of a PEH geometry on its energy harvesting performance in a real-world setting by considering field health monitoring data of a large-scale cable-stayed bridge in NSW, Australia. A shape optimisation framework is developed based on acceleration events, i.e., where the level of response exceeds a certain threshold. Designs obtained in the event-based optimisation are then clustered to propose several best candidates for continuous energy generation. This work represents the first study on PEH design optimisation for real operational conditions. [ABSTRACT FROM AUTHOR]

Featured Application: Unconventional, highly transparent building integrated photovoltaics. We report on the field testing datasets and performance evaluation results obtained from a commercial property-based visually-clear solar window installation site in Perth-Australia. This installation was fitted into a refurbished shopping center entrance porch and showcases the potential of glass curtain wall-based solar energy harvesting in built environments. In particular, we focus on photovoltaic (PV) performance characteristics such as the electric power output, specific yield, day-to-day consistency of peak output power, and the amounts of energy generated and stored daily. The dependencies of the generated electric power and stored energy on multiple environmental and geometric parameters are also studied. An overview of the current and future application potential of high-transparency, visually-clear solar window-based curtain wall installations suitable for practical building integration is provided. [ABSTRACT FROM AUTHOR]

The article presents a summary of five research projects on different biomass harvesting systems in Australian plantations. The first trial assessed the productivity and cost of slash-bundling the harvesting residues in clear felled area using Pinox slash-bundler in Eucalypt plantation in Tasmania. Second project investigated the efficiency of a European mobile chipper to collect pine harvesting residues Green Triangle (Victoria) while in the third project a conventional forwarder was studied to recover pine residues logs (called Fibre plus material) as an integrated biomass operation in Western Australia. The product quality and fuel consumption of the biomass harvesting systems were also assessed within the trials. Whole tree biomass harvesting (including feller-buncher, grapple skidder and chipper) was another trial carried out in low-productivity Eucalypt stands in Western Australia to produce biomass chips for bioenergy purposes. Where bioenergy markets are strong, thinning materials (e.g. large branches) may also be utilised as a source for bioenergy. The operating costs and environmental impacts (including remaining residues assessment to sustain soil quality in biomass recovery operations) of different technologies were compared/discussed in this article. [ABSTRACT FROM AUTHOR]