1. Molecular confirmation of pathological specimen integrity in Australasia.
- Author
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Bell CG, Wood DR, Cheong SJ, Kwan E, Sinosich M, Delprado W, and Baumgart KW
- Subjects
- Australasia, Biopsy, Forensic Medicine methods, Humans, Microsatellite Repeats, Pathology, Surgical standards, Polymorphism, Single Nucleotide, Specimen Handling standards, DNA Fingerprinting methods, Medical Errors, Pathology, Surgical methods, Patient Identification Systems methods, Specimen Handling methods
- Abstract
Aim: Investigations into 14 suspected pathology sample identification errors and mix-ups were performed, as a service for several public hospital and private laboratories, from 2005 to 2007., Methods: Analyses were performed with the forensic ABI Identifiler kit of 16 microsatellites (15 autosomal and amelogenin) on DNA from paraffin-embedded tissues or blood specimens and compared to independently verified (single or multiple) patient samples., Results: Of 23 unique patient specimens referred for sample integrity confirmation because of pathologist, clinician or patient concern, six (26.1%) were demonstrated to be discordant, indicating that specimen identification errors or mix-ups had occurred., Conclusions: Due to their great sensitivity and high discrimination power, forensic identity multiplex systems using either microsatellites or single nucleotide polymorphisms (SNPs) can resolve concerns about pathology specimen identity and integrity.
- Published
- 2009
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