1. Molecular diversity of fungal and bacterial communities in the marine sponge Dragmacidon reticulatum.
- Author
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Passarini MR, Miqueletto PB, de Oliveira VM, and Sette LD
- Subjects
- Actinobacteria classification, Actinobacteria genetics, Actinobacteria isolation & purification, Animals, Ascomycota classification, Ascomycota genetics, Ascomycota isolation & purification, Atlantic Ocean, Bacteria isolation & purification, Bacteroidetes classification, Bacteroidetes genetics, Bacteroidetes isolation & purification, Basidiomycota classification, Basidiomycota genetics, Basidiomycota isolation & purification, Chloroflexi classification, Chloroflexi genetics, Chloroflexi isolation & purification, Cyanobacteria classification, Cyanobacteria genetics, Cyanobacteria isolation & purification, DNA, Bacterial genetics, DNA, Ribosomal genetics, Fungi isolation & purification, Gene Library, Genes, rRNA, Phylogeny, Proteobacteria classification, Proteobacteria genetics, Proteobacteria isolation & purification, RNA, Ribosomal, 16S genetics, Seawater microbiology, Sequence Analysis, DNA, Bacteria classification, Bacteria genetics, Biodiversity, Fungi classification, Fungi genetics, Porifera microbiology
- Abstract
The present work aimed to investigate the diversity of bacteria and filamentous fungi of southern Atlantic Ocean marine sponge Dragmacidon reticulatum using cultivation-independent approaches. Fungal ITS rDNA and 18S gene analyses (DGGE and direct sequencing approaches) showed the presence of representatives of three order (Polyporales, Malasseziales, and Agaricales) from the phylum Basidiomycota and seven orders belonging to the phylum Ascomycota (Arthoniales, Capnodiales, Dothideales, Eurotiales, Hypocreales, Pleosporales, and Saccharomycetales). On the other hand, bacterial 16S rDNA gene analyses by direct sequencing approach revealed the presence of representatives of seven bacterial phyla (Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Lentisphaerae, Chloroflexi, and Planctomycetes). Results from statistical analyses (rarefaction curves) suggested that the sampled clones covered the fungal diversity in the sponge samples studied, while for the bacterial community additional sampling would be necessary for saturation. This is the first report related to the molecular analyses of fungal and bacterial communities by cultivation-independent approaches in the marine sponges D. reticulatum. Additionally, the present work broadening the knowledge of microbial diversity associated to marine sponges and reports innovative data on the presence of some fungal genera in marine samples., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
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