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Five global monthly top-of-atmosphere (TOA) outgoing longwave radiation (OLR) products are evaluated in this study, including the products derived from the High-Resolution Infrared Radiation Sounder (HIRS), Clouds and the Earth's Radiant Energy System (CERES), Advanced Very High Resolution Radiometer (AVHRR), the CM SAF cLoud, Albedo and surface RAdiation dataset from AVHRR data (CLARA), and the Global Energy and Water Cycle EXchanges (GEWEX) project. Results show that overall there is good consistency among these five products. Larger differences are found between GEWEX and CERES (HIRS) after (before) 2000 (RMSE ~ 5 W/m2), particularly in the tropical regions. In terms of global mean values, GEWEX shows large differences with the other products from the year 1992 to 2002, and CLARA shows large differences from the year 1979 to 1981, which are more obvious in the global ocean values. Large discrepancies among these products exist at low latitudinal bands, particularly before the year 2000. Australia and Asia (mid–low latitude part) are two typical regions in which larger differences are found. [ABSTRACT FROM AUTHOR]

Valsa canker of apple (VCA) caused by Valsa mali severely affected apple production in east Asia. With the increase in drug resistance, there is an urgent need for efficient and environmentally friendly antifungal agents. Coumarins have attracted much attention due to their excellent antimicrobial activity against plant pathogens. In this study, the antifungal activity of several coumarins against phytopathogenic fungi was evaluated, and then the antifungal activity of the screened 6-MCM against V. mali and its underlying mechanism was further investigated. The results of the in vitro antifungal activity assay showed that some coumarins had significant inhibitory effects on V. mali. Notably, 400 mg/L of 6-MCM had the best antifungal activity of 94.6%. Further experiments showed that 6-MCM slowed down the growth of V. mali mycelia and the germination of spores in a concentration-dependent manner, with EC50 of 185.49 and 54.62 mg/L, respectively. In addition, 6-MCM treatment increased mycelial conductivity, extracellular protein leakage, and MDA content, resulting in damage to the cell membrane. Moreover, 6-MCM significantly reduced the cell wall degrading enzymes secreted by V. mali, including EG, PG and PL, thereby limiting its pathogenic capacity. SEM and TEM results showed that 6-MCM treatment had a significant effect on the morphology and ultrastructure of mycelial cells. Inoculation of isolated apple branches found that the application of 6-MCM effectively inhibited the development of VCA and significantly reduced the incidence. All these results suggest that 6-MCM has the potential as a green substitute for VCA control. [ABSTRACT FROM AUTHOR]

Role of avian hosts in shaping persistence, evolution, and dispersal of global low pathogenic avian influenza virus (LPAIV) H9N2 remains uncertain. Under Bayesian Markov Chain Monte Carlo framework, we used the discrete trait analysis (DTA) to reconstruct host and location switches in the evolutionary history of global H9N2 given hemagglutinin gene sequences from 18 countries/regions between 1976 and 2018. We employed generalized linear models (GLMs) to inform virus migration rates by empirical predictors. Global H9N2 isolates were mostly sampled from domestic Phasianidae in low- and middle-income countries with poor bio-security. Anatidae was inferred as the ancestral source from which the virus spread to domestic waterfowl, and later to domestic Phasianidae who have become the dominant host to sustain the virus, especially in Asia. Poultry trade was a well-supported driver to H9N2 spread across countries/regions. Strict bio-security and separation between wild and domestic poultry can be used to mitigate virus spread. • Bayesian phylodynamic inferences revealed Anatidae as the ancestral source of global H9N2. • The reconstructed host transitions as H9N2 spreading from wild Anatidae to domestic waterfowl, and later to domestic Phasianidae. • Phasianidae becomes the dominant host in recent years with a longer reward time, especially in Asia. • Poultry trade and sharing a border contribute to H9N2 spread across countries/regions [ABSTRACT FROM AUTHOR]

Model-based phylodynamic approaches recently employed generalized linear models (GLMs) to uncover potential predictors of viral spread. Very recently some of these models have allowed both the predictors and their coefficients to be time-dependent. However, these studies mainly focused on predictors that are assumed to be constant through time. Here we inferred the phylodynamics of avian influenza A virus H9N2 isolated in 12 Asian countries and regions under both discrete trait analysis (DTA) and structured coalescent (MASCOT) approaches. Using MASCOT we applied a new time-dependent GLM to uncover the underlying factors behind H9N2 spread. We curated a rich set of time-series predictors including annual international live poultry trade and national poultry production figures. This time-dependent phylodynamic prediction model was compared to commonly employed time-independent alternatives. Additionally the time-dependent MASCOT model allowed for the estimation of viral effective sub-population sizes and their changes through time, and these effective population dynamics within each country were predicted by a GLM. International annual poultry trade is a strongly supported predictor of virus migration rates. There was also strong support for geographic proximity as a predictor of migration rate in all GLMs investigated. In time-dependent MASCOT models, national poultry production was also identified as a predictor of virus genetic diversity through time and this signal was obvious in mainland China. Our application of a recently introduced time-dependent GLM predictors integrated rich time-series data in Bayesian phylodynamic prediction. We demonstrated the contribution of poultry trade and geographic proximity (potentially unheralded wild bird movements) to avian influenza spread in Asia. To gain a better understanding of the drivers of H9N2 spread, we suggest increased surveillance of the H9N2 virus in countries that are currently under-sampled as well as in wild bird populations in the most affected countries. [ABSTRACT FROM AUTHOR]

ABSTRACT This study evaluates the simulation of the spring Arctic Oscillation ( AO)-western North Pacific linkage based on the 16 state-of-the-art climate models from the Coupled Model Intercomparison Project Phase 5. The validation focuses on the predominant process connecting the spring AO with the East Asian summer monsoon: the formation and persistence of the spring AO-associated cyclonic anomaly over western North Pacific ( WPCA) from spring to summer. The results indicate that 8 of 16 models can reproduce both the formation and persistence of the WPCA. Because the formation of the WPCA is directly related to the existence of the spring upper-level North Pacific atmospheric dipole ( NPAD), the analyses suggest that a given model can reproduce the spring AO-associated NPAD if the model is capable of simulating the spring AO-associated deceleration of the subtropical westerly jet and the transient eddy activities around the westerly jet exit. Furthermore, the westerly jet anomalies are closely related to the simulated mean state of the westerly jet and the AO Pacific component, which could be further attributed to the simulated sea surface temperature biases over the equatorial Western Pacific. [ABSTRACT FROM AUTHOR]

Objective Previous genome-wide association studies (GWAS), which were mainly based on single-variant analysis, have identified many systemic lupus erythematosus (SLE) susceptibility loci. However, the genetic architecture of this complex disease is far from being understood. The aim of this study was to investigate whether using a gene-based analysis may help to identify novel loci, by considering global evidence of association from a gene or a genomic region rather than focusing on evidence for individual variants. Methods Based on the results of a meta-analysis of 2 GWAS of SLE conducted in 2 Asian cohorts, we performed an in-depth gene-based analysis followed by replication in a total of 4,626 patients and 7,466 control subjects of Asian ancestry. Differential allelic expression was measured by pyrosequencing. Results More than one-half of the reported SLE susceptibility loci showed evidence of independent effects, and this finding is important for understanding the mechanisms of association and explaining disease heritability. ANXA6 was detected as a novel SLE susceptibility gene, with several single-nucleotide polymorphisms (SNPs) contributing independently to the association with disease. The risk allele of rs11960458 correlated significantly with increased expression of ANXA6 in peripheral blood mononuclear cells from heterozygous healthy control subjects. Several other associated SNPs may also regulate ANXA6 expression, according to data obtained from public databases. Higher expression of ANXA6 in patients with SLE was also reported previously. Conclusion Our study demonstrated the merit of using gene-based analysis to identify novel susceptibility loci, especially those with independent effects, and also demonstrated the widespread presence of loci with independent effects in SLE susceptibility genes. [ABSTRACT FROM AUTHOR]

This study explores the impacts a presale contract has on a developer's pricing and production decisions in a gametheoretical framework. In an environment where developers have full capital market access, the findings reveal that both developers and buyers are indifferent between a presale and a spot sale method. However, in an environment with financing constraints, both developers and buyers are better off when a presale method is used. This is because the presale method solves the financing constraint by injecting equity into the development and, hence, reducing financing costs. This model prediction seems to describe well the real world situations seen in some of the property markets in Asia that have nascent financial systems. [ABSTRACT FROM AUTHOR]

The thermal structure of the environmental atmosphere associated with Terrestrial Gamma-ray Flashes (TGFs) is investigated with the combined observations from several detectors (FERMI, RHESSI, and Insight-HXMT) and GNSS-RO (SAC-C, COSMIC, GRACE, TerraSAR-X, and MetOp-A). The geographic distributions of TGF-related tropopause altitude and climatology are similar. The regional TGF-related tropopause altitude in Africa and the Caribbean Sea is 0.1–0.4 km lower than the climatology, whereas that in Asia is 0.1–0.2 km higher. Most of the TGF-related tropopause altitudes are slightly higher than the climatology, while some of them have a slightly negative bias. The subtropical TGF-producing thunderstorms are warmer in the troposphere and have a colder and higher tropopause over land than the ocean. There is no significant land–ocean difference in the thermal structure for the tropical TGF-producing thunderstorms. The TGF-producing thunderstorms have a cold anomaly in the middle and upper troposphere and have stronger anomalies than the deep convection found in previous studies. [ABSTRACT FROM AUTHOR]

Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) have revolutionized pest control, but the benefits of this approach have been reduced by the evolution of resistance in pests. The widely adopted 'pyramid strategy' for delaying resistance entails transgenic crops producing two or more distinct toxins that kill the same pest. The limited experimental evidence supporting this strategy comes primarily from a model system under ideal conditions. Here we tested the pyramid strategy under nearly worst-case conditions, including some cross-resistance between the toxins in the pyramid. In a laboratory selection experiment with an artificial diet, we used Bt toxins Cry1Ab, Cry1F, and Cry1Ie singly or in pairs against Ostrinia furnacalis, one of the most destructive pests of corn in Asia. Under the conditions evaluated, pairs of toxins did not consistently delay the evolution of resistance relative to single toxins. [ABSTRACT FROM AUTHOR]

Background: Robust phylogenies for species with giant genomes and closely related taxa can build evolutionary frameworks for investigating the origin and evolution of these genomic gigantisms. Paris japonica (Melanthiaceae) has the largest genome that has been confirmed in eukaryotes to date; however, its phylogenetic position remains unresolved. As a result, the evolutionary history of the genomic gigantisms in P. japonica remains poorly understood. Results: We used next-generation sequencing to generate complete plastomes of P. japonica, P. verticillata, Trillium govanianum, Ypsilandra thibetica and Y. yunnanensis. Together with published plastomes, the infra-familial relationships in Melanthiaceae and infra-generic phylogeny in Paris were investigated, and their divergence times were calculated. The results indicated that the expansion of the ancestral genome of extant Paris and Trillium occurred approximately from 59.16 Mya to 38.21 Mya. The sister relationship between P. japonica and the section Euthyra was recovered, and they diverged around the transition of the Oligocene/Miocene (20 Mya), when the Japan Islands were separated from the continent of Asia. Conclusions: The genome size expansion in the most recent common ancestor for Paris and Trillium was most possibly a gradual process that lasted for approximately 20 million years. The divergence of P. japonica (section Kinugasa) and other taxa with thick rhizome may have been triggered by the isolation of the Japan Islands from the continent of Asia. This long-term separation, since the Oligocene/Miocene boundary, would have played an important role in the formation and evolution of the genomic gigantism in P. japonica. Moreover, our results support the taxonomic treatment of Paris as a genus rather than dividing it into three genera, but do not support the recognition of T. govanianum as the separate genus Trillidium. [ABSTRACT FROM AUTHOR]

• The incidence of Clostridioides difficile infection is increasing in developing regions such as Asia. • Clinical diagnosis and routine monitoring of CDI in underdeveloped regions hampered by high cost of diagnostic reagents. • We found a novel target for the identification of hypervirulent Clostridioides difficile Sequence Type 1. • We developed a highly specific and sensitive primer set for simultaneous identification of hypervirulent ST1 and detection of toxin genes directly on total fecal DNA. The incidence of Clostridioides difficile infection and the prevalence of hypervirulent ST1 (BI/NAP1/027) strain are increasing, especially in developing countries. We aimed to develop a new PCR assay for the identification of hypervirulent ST1 strains and toxigenic C. difficile in stool samples. We established a quadruplex TaqMan real-time PCR (pilW _4-plex PCR) assay targeting the pilW , a ST1-specific type Ⅳ minor pilin gene, and three C. difficile genes including cdtB , tcdB , and hsp. The sensitivity and specificity of the assay was tested using 403 C. difficile isolates and 180 unformed stool sample. The results were compared with anaerobic culture-based conventional PCR method and MLST. The pilW _4-plex PCR identified toxigenic C. difficile in 333 (82.6%, 333/403) isolates with 100% sensitivity and specificity, and in 78 (43.3%, 78/180) stool samples with the sensitivity and specificity of 94.7% and 93.3%, respectively. Hypervirulent ST1 were detected in 21 strains and nine stool samples by the pilW _4-plex PCR. The pilW _4-plex PCR assay has no cross-reaction with non-toxigenic C. difficile or other bacteria. The pilW _4-plex PCR assay is an accurate and rapid method with high sensitivity and specificity for identification of ST1 and detection of toxigenic C. difficile in stool. [ABSTRACT FROM AUTHOR]

Domestic sheep and their wild relatives harbor substantial genetic variants that can form the backbone of molecular breeding, but their genome landscapes remain understudied. Here, we present a comprehensive genome resource for wild ovine species, landraces and improved breeds of domestic sheep, comprising high-coverage (∼16.10×) whole genomes of 810 samples from 7 wild species and 158 diverse domestic populations. We detected, in total, ∼121.2 million single nucleotide polymorphisms, ∼61 million of which are novel. Some display significant (P < 0.001) differences in frequency between wild and domestic species, or are private to continent-wide or individual sheep populations. Retained or introgressed wild gene variants in domestic populations have contributed to local adaptation, such as the variation in the HBB associated with plateau adaptation. We identified novel and previously reported targets of selection on morphological and agronomic traits such as stature, horn, tail configuration, and wool fineness. We explored the genetic basis of wool fineness and unveiled a novel mutation (chr25: T7,068,586C) in the 3'-UTR of IRF2BP2 as plausible causal variant for fleece fiber diameter. We reconstructed prehistorical migrations from the Near Eastern domestication center to South-and-Southeast Asia and found two main waves of migrations across the Eurasian Steppe and the Iranian Plateau in the Early and Late Bronze Ages. Our findings refine our understanding of genome variation as shaped by continental migrations, introgression, adaptation, and selection of sheep., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)

Introduction: Despite acupuncture having been successfully used for the clinical treatment for vascular dementia in Asian countries for centuries, scientifically rigorous evidence is lacking for standardizing therapeutic parameters. To address this problem, it is necessary to examine the parameters of acupuncture using scientific methodology. The goal of this study is to investigate various therapeutic parameters, including manipulation, retention, and frequency of acupuncture, and their contribution to the efficacy of acupuncture in VD., Methods: We selected needle retention, treatment frequency, and needle rotation as the parameters. Acupuncture was performed on acupoints ST36 and GV20. Morris Water Maze was selected to assess the effect of acupuncture on cognitive function and Nissl staining indicated the hippocampal neuronal damage in VD rats., Results: Acupuncture stimulation resulted in a significant improvement in the spatial learning and memory deficit and reversed neuronal damage in the hippocampus. The approach of needle retention with 10 min, rotation for 30s every 5 min or daily treatment with acupuncture was more effective than nonretention, nonrotation, or alternative day treatment group., Conclusion: This study demonstrated that needle retention, treatment frequency, and needle manipulation are important factors in improving the learning and memory as well as reducing the neuronal damage of the hippocampus in a rat VD model. These findings strongly suggest that the appropriate therapeutic parameters could significant influence the efficiency in animal experiments., (© 2020 The Authors. Brain and Behavior published by Wiley Periodicals LLC.)