1. The comparative study revealed that the hTERT-CSF cell line was the most susceptible cell to the Lumpy skin disease virus infection among eleven cells.
- Author
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Ma, Chunling, Ren, Shanhui, Afera, Tadele Berihun, Yang, Xue, Lin, Yugang, Gao, Xiaohong, Wang, Fangping, Qiu, Xusheng, Wang, Xiangwei, Yin, Xiangping, Sun, Yuefeng, Wan, Xuerui, and Chen, Hao-tai
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LUMPY skin disease , *VIRUS diseases , *CELL lines , *TISSUE culture , *VACCINE development , *PLANT propagation - Abstract
Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in Asia, including China. Improving the propagation of LSDV is important for diagnostics and vaccine production. Our study identified and compared the LSDV susceptibility of eleven standard cells using western blot, indirect immune-fluorescence assay, quantitative PCR, and 50 % tissue culture infectious dose. Our finding revealed that the LSDV strain could infect five cell lines and show a cytopathic effect. Furthermore, the hTERT-CSF cell line had the highest level of virus in the five cell models, followed by BHK-21, MDBK, Vero, and hTERT-ST. Hence, hTERT-CSF could be used as a candidate cell line for basic and applied research, clinical application, and LSDV vaccine development, providing a vital reference in LSDV and other viruses. • The LSDV could infect five cell lines, including hTERT-CSF, BHK21, MDBK, Vero, and hTERT-ST. • The hTERT-CSF cell line was the most suitable for LSDV infection among the five cell models. • The hTERT-CSF could be used as a candidate cell line for basic and applied research, clinical application, and LSDV vaccine development. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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