1. Linking traditional anti-ulcer use of rhizomes of Bergenia ciliata (Haw.) to its anti-Helicobacter pylori constituents.
- Author
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Ali E, Arshad N, Bukhari NI, Nawaz Tahir M, Zafar S, Hussain A, Parveen S, Qamar S, Shehzadi N, and Hussain K
- Subjects
- Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents pharmacology, Asia, Benzopyrans therapeutic use, Humans, Medicine, Traditional, Microbial Sensitivity Tests, Plant Extracts chemistry, Plant Extracts pharmacology, Stomach Ulcer drug therapy, Benzopyrans isolation & purification, Helicobacter pylori drug effects, Rhizome, Saxifragaceae chemistry, Ulcer drug therapy
- Abstract
The rhizomes of Bergenia ciliata ( B. ciliata , Family: Saxifragaceae ) are widely used for treating gastric ulcers in folk medicine in Asia. It was hypothesized that anti-ulcer activity of B. ciliata is due to its anti-Helicobacter pylori ( H. pylori) activity. The anti-H. pylori activity was investigated on six clinical bacterial isolates using agar well-diffusion and broth micro-dilution methods. The anti-H. pylori activity of amoxicillin (standard) was the highest (Zone of inhibition; ZI = 25 mm, minimum inhibitory concentration; MIC=0.125 µg/µL) whereas among all the extracts of the rhizomes, methanol extract showed the highest activity (ZI = 16 mm, MIC = 12.50 µg/µL). Bioassay guided isolation of methanol extract using chromatographic and crystallization techniques isolated bergenin (ZI = 21mm, MIC = 0.391µg/µL) as constituent responsible for anti-H. pylori activity. The present study describes for the first time anti-H. pylori activity and possible mechanism of anti-ulcer properties of rhizomes of B. ciliata.
- Published
- 2020
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