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Background: Microsatellite instability (MSI) has been a hot topic in cancer research. Determining MSI status greatly aids tumor prognosis and treatment plans. However, MSI data for Asian cancer patients with prognostic information are scarce. Here, our aim was to clarify MSI status and its prognostic value in a large Chinese cohort with different tumors. Patients and Methods: Tissue samples from 600 Chinese cases, including 150 endometrial cancers, 150 colorectal cancers, 150 liver cancers and 150 gastric cancers, were used for IHC and MSI examinations. Two mononucleotide and three dinucleotide markers were used to analyze MSI status. Results: In total,17.3% (26/150) of endometrial cancer patients showed positive MSI,10.0% (15/150) in colorectal cancer, 2.7% (4/150) in liver cancer, and 2.7% (4/150) in gastric cancer. Tumor location (P < 0.001 for colorectal cancer) and clinical stage (P =0.038 for gastric cancer) showed significant correlations with MSI status in gastrointestinal carcinogenesis. The mismatch repair (MMR) deficiency was observed in 20 colorectal cases (13.3%) and was significantly more frequent in the MSI-positive group (P < 0.001). Interestingly, the prevalence of MSI-H was mostly occurred in early-stage tumors, and none was in late stage (stage IV). Meanwhile, low clinicopathological stage had significant correlation with longer survival in multiple cancers here. Conclusion: The incidence of microsatellite instability varies among different cancer types. And the prevalence of MSI-H mostly occurred early clinicopathological stage. In addition, our study provided a large Asian cohort screened by five loci PCR method and significantly increased knowledge on the prognostic significance of MSI in Asia. [ABSTRACT FROM AUTHOR]

Building on the under-utilized power of case studies, this conceptual review considers information to enhance Asian tourism sites and attractions. Soft infrastructure, defined as the total effect of the hospitality, interpretive and person-to-person encounters, was considered at 16 strategically selected locations drawn from 7 Asian countries. An emic, tourist-oriented perspective was used to appraise the effects of the soft infrastructure. The researchers employed information from academic publications, attraction websites, tourists' reviews and personal visits to identify common cross-site issues. Seven key issues were identified: the importance of emotional labour/sincerity; the value of quality and professionalism; creating understanding and the role of language; the appeal of performance and spectacle; the usefulness of immersion and participation; the power of authentic local voices; and the management of crowding and close encounters. Site and tourist precinct attraction managers can evaluate these issues to better use their tourism assets and create enhanced on-site experiences. [ABSTRACT FROM AUTHOR]