1. Genotoxic evaluation of the biocomponents of the cricket, Gryllus bimaculatus, using three mutagenicity tests.
- Author
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Ahn MY, Bae HJ, Kim IS, Yoo EJ, Kwack SJ, Kim HS, Kim DH, Ryu KS, Lee HS, Kim JW, Kim I, and Lee BM
- Subjects
- Animals, Bone Marrow Cells drug effects, Bone Marrow Cells pathology, CHO Cells, Chromosome Aberrations, Cricetinae, Cricetulus, Asia, Eastern, Gryllidae metabolism, Male, Medicine, East Asian Traditional, Mice, Mice, Inbred ICR, Micronucleus Tests, Salmonella typhi drug effects, Salmonella typhi genetics, Gryllidae chemistry, Insect Proteins toxicity
- Abstract
The mutagenic potential of the extracted components of Gryllus bimaculatus, a species of cricket, was evaluated using short-term genotoxicity tests including the Ames, chromosome aberration, and micronuclei tests. In a Salmonella typhimurium assay, G. bimaculatus extract did not produce any mutagenic response in the absence or presence of S9 mix with TA98, TA100, TA1535, and TA1537. Chromosome aberration testing showed that G. bimaculatus had no significant effect on Chinese hamster ovary (CHO) cells. In the mouse micronucleus test, no significant alteration in occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice intraperitoneally administered with G. bimaculatus extract at doses of 15, 150, or 1500 mg/kg. These results indicate that G. bimaculatus extract exerts no mutagenic effect in these in vitro and in vivo systems.
- Published
- 2005
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