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Objective. To determine the prevalence of sexual initiation and the contraceptive practices of adolescents in the tri-border area between the cities of Foz do Iguaçu (Brazil), Ciudad del Este (Paraguay), and Puerto Iguazú (Argentina). Method. A cross-sectional study was conducted with 2 788 adolescents from public schools (age 12 to 18 years). Information was obtained through questionnaires administered in the schools between 2012 and 2013. The prevalence of sexual initiation, contraceptive practices, and sociodemographic variables was analyzed. To verify the association between the studied variables, the chi-square test was used with a significance level of 5%. Results. The overall prevalence of sexual initiation among adolescents was 34.6% (Puerto Iguazú: 45.3%; Foz do Iguaçu: 35.2%; Ciudad del Este: 23.3%). The prevalence of sexual initiation was higher in males and in the 15-16 year age range. Sexual initiation was associated with sex, age, schooling, and school shift (morning, afternoon, or evening). Having received information on contraceptives was associated with sex, age, schooling, and contraceptive use in all three cities/countries. Adolescents of both sexes had received information about contraceptives, especially from the family and the school. Conclusions. It is suggested that municipalities/countries seek to structure a tri-national border policy with intersectoral programs and projects in order to promote educational practices regarding sexual and reproductive health for adolescents. [ABSTRACT FROM AUTHOR]