1. Seroreactivity to HIV-1 V3 subtypes A to H peptides of Argentinian HIV-positive sera.
- Author
-
Devito C, Levi M, Hinkula J, Fernandez Medina RD, Libonatti O, and Wigzell H
- Subjects
- Amino Acid Sequence, Argentina epidemiology, HIV Antibodies blood, HIV Envelope Protein gp120 immunology, HIV Seropositivity epidemiology, HIV Seropositivity immunology, HIV-1 immunology, Humans, Immunoglobulin G immunology, Molecular Sequence Data, Peptide Fragments immunology, Substance Abuse, Intravenous, HIV Antibodies classification, HIV Envelope Protein gp120 classification, HIV Seropositivity virology, HIV-1 classification, Peptide Fragments classification
- Abstract
Serologic assays could be useful for determining circulating subtypes in different geographic regions. A total of 175 serum samples from the same number of Argentinian HIV-infected patients from Buenos Aires and Rosario were tested against a panel of peptides representing V3 consensus subtypes A through H. A V3 peptide enzyme immunoassay was used for screening the sera. Most sera were reactive with peptides representing subtypes B (58.28%), F (13.14%), and A (8.57%). Cross-reactivity between the remainder of the peptides was observed. Genotypes of eight patients from Rosario were determined and compared with serotyping. Results showed that seven of eight genotyped patients reacted with their respective consensus B peptide and one reacted with consensus B and F. V3 peptide serology proved to be useful for determining HIV-1 clades circulating in Argentina.
- Published
- 1998
- Full Text
- View/download PDF