Search

A novel bacterium, designated JB02H27 T , was isolated from marine sediment collected from the southern Scott Coast, Antarctica. Cells were Gram-stain-negative, facultatively anaerobic, polar-flagellated and motile rods. Growth occurred at 4-45 °C, at pH 7.0-9.0 and with 3-25 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain JB02H27 T consistently fell within the genus Marinobacter and formed a clade together with Marinobacter algicola DG893 T (98.8 % similarity), Marinobacter confluentis KCTC 42705 T (98.4 %), Marinobacter salarius R9SW1 T (98.4%) and Marinobacter halotolerans CP12 T (97.9 %), which were subsequently used as reference strains for comparisons of phenotypic and chemotaxonomic characteristics. Average nucleotide identity values between strain JB02H27 T and the four related type strains were 80.9, 76.6, 81.9 and 76.3 %, respectively. The major fatty acids were summed feature 3, C 16 : 0 , C 18 : 1 ω 9 c and C 16 : 0  N alcohol. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified phospholipid, aminolipid, aminophospholipid and glycolipids. The sole respiratory quinone was ubiquinone-9. The DNA G+C content was 56.9 mol%. Based on the genomic, phylogenetic, phenotypic and chemotaxonomic analysis, we propose that strain JB02H27 T represents a novel species of the genus Marinobacter , for which the name Marinobacter denitrificans sp. nov. is proposed. The type strain is JB02H27 T (=GDMCC 1.1528 T =KCTC 62941 T ).

Polyphasic taxonomic analysis was performed to characterize a novel bacterium, which was isolated from surface sediment of the Terra Nova Bay, Antarctica, and designated as R04H25 T . The cells of the isolate were Gram-stain-negative, aerobic, non-motile, slightly curved rods. Growth occurred at 4-42 °C, pH 7.0-9.5, and in 1-15 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences indicated that strain R04H25 T formed an independent lineage within the genus Pseudidiomarina and its nearest neighbours were Pseudidiomarina donghaiensis 908033 T (98.2 %), Pseudidiomarina marina PIM1 T (98.1 %), Pseudidiomarina woesei W11 T (97.8 %), Pseudidiomarina maritima 908087 T (97.1 %) and Pseudidiomarina tainanensis PIN1 T (97.0 %). The average nucleotide identities between strain R04H25 T and the nearest neighbours were 76.2-77.7 %. The major fatty acids were iso-C 17 : 0 , summed feature 9, iso-C 15 : 0 , C 16 : 0 and iso-C 11 : 0 3-OH. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid, three unidentified glycolipids and two unidentified lipids. The predominant respiratory quinone was ubiquinone 8. The genomic DNA G+C content was 48.2 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic results, we propose a novel species named as Pseudidiomarina gelatinasegens sp. nov. in the genus Pseudidiomarina , with the type strain R04H25 T (=GDMCC 1.1503 T =KCTC 62911 T ).