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The wide variety of uses for nanoparticles (NPs) is due to their unique combination of features in a single assembly. The arc melted copper-cobalt ingot sample were qualitatively studied using laser induced breakdown spectroscopy (LIBS). Later, using the fabricated alloy as a target material for Nd:YAG laser ablation, CuCo2O4 NPs were synthesized. The magnetic properties of the synthesized NPs were studied using a vibrating sample magnetometer (VSM). To determine the composition and morphology of the synthesized NPs, X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and dynamic light scattering (DLS) techniques were used. The TEM and DLS showed that particles were spherical in shape with an average size of 32 nm and 28 nm, respectively. The antibacterial activity of the synthesized NPs was studied against S. aureus and E. coli strains as positive and negative controls using a standard approach. CuCo2O4 nanoparticles exhibited non-mutagenic potential against S. typhimurium TA-98 and TA-100 strains. Furthermore, the magnetic hyperthermia study of CuCo2O4 nanofluid was examined using a lab-made apparatus. The specific absorption rates (SAR) of 4.57 and 5.17 W/g were determined for the magnetic field strength of 230 μT and 247 μT, respectively. The study shows antibacterial activity and magnetic hyperthermia potential of the synthesized nanoparticles. [ABSTRACT FROM AUTHOR]