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The increase in international trade due to globalization is evident in southeast Spain, which has become the top exporter of fruit and vegetables. Countries within the European Union, such as Germany and France, emphasize the sustainability and environmental impacts of these products. Hence, a greater understanding of the environmental implications of transporting fruit and vegetables between their origin and their destination might improve the sustainability of this commercial activity. The concept of a carbon footprint is a recognized environmental indicator that can be used for life cycle analysis. Here, a rigorous carbon footprint assessment was developed to examine the impact of using cardboard box containers to store and transport 1,000 t of fruit and vegetable products by road from their origin in Almería, Spain, to a destination market. The assessment included the fabrication of the cardboard boxes, the service they provide while transporting the products to the distribution center of the destination, and the end-of-life of the boxes for the six main products grown in Almería. The results showed that storing and transporting 1,000 t of product by road emits between 58 t and 130 t of CO 2 e depending on the fruit or vegetable type and the destination market. The implications of the end-of-life scenarios with respect to the destination are also discussed. Furthermore, a sensitivity analysis was conducted for the transport distance. Lastly, biogenic CO 2 production was also assessed according to standard carbon footprint assessment method. • The carbon footprint of storage and transport of fruit and vegetables was assessed as an environmental indicator. • End-of-life scenarios and transport distance were the key aspects affecting the environmental impact. • Storing and transporting 1,000 t of fruits from Almería to main European markets emits between 58 t and 130 t of CO 2 e. [ABSTRACT FROM AUTHOR]