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This paper investigated the chemical composition of the essential oil (EO) of Origanum floribundum Munby. by gas chromatography coupled with mass spectrometry and evaluated its antimicrobial activity. The compositional analysis identified 33 compounds representing 97.26% of the total composition, of which the majority component was carvacrol at 41.092%. The antimicrobial activity evaluated deduced that O. floribundum EO affects all tested strains, of which the most susceptible were Staphylococcus aureus and Candida albicans, with growth inhibition zones of 25.83 mm and 23.25 mm, respectively. A bactericidal effect was recorded on Staphylococcus aureus and Bacillus cereus at 1.56 mg/mL and 3.125 mg/mL EO, respectively, and a fungistatic effect on Candida albicans at 1.56 mg/mL of EO. The intestinal microbiota strains, Bifidobacterium animalis subsp lactis and Lactobacillus rhamnosus LbRE-LSAS, were not affected by O. floribundum EO. [ABSTRACT FROM AUTHOR]

Objectives: This study investigated the in vitro effect of Origanum glandulosum, Ammoides verticillata, and Saccocalyx satureioides essential oils against planktonic cells and biofilms formed by single and mixed species of Candida albicans and Candida glabrata oral isolates. The effect of the essential oils in combination with amphotericin B on planktonic cells was also studied., Design: The antifungal susceptibility of planktonic cells was evaluated by disc diffusion and broth microdilution methods. Resazurin assay and scanning electron microscopy (SEM) were employed to determine the antibiofilm activity. The combinatory effect was evaluated by the checkerboard method. Essential oils were characterized by gas chromatography-mass spectrometry (GC-MS)., Results: The minimal inhibitory concentrations (MICs) and the minimum fungicidal concentrations (MFCs) of the studied essential oils were ranged between 250 and 2000 µg/mL. Biofilms were inhibited and eradicated by the essential oils at sub-inhibitory concentrations of 500 and 1000 µg/mL, respectively. SEM studies revealed a reduction in the preformed biofilm as a result of Origanum glandulosum essential oil treatment for single and mixed biofilms. Synergistic activity was found when Origanum glandulosum essential oil was combined with amphotericin B against Candida albicans. GC-MS analysis revealed that thymol was the major compound in Origanum glandulosum (38.36 %) and Ammoides verticillata (48.99 %) essential oils, while Saccocalyx satureioides essential oil was dominated by borneol (27.36 %)., Conclusions: The studied essential oils showed significant antifungal and antibiofilm activities, which support their effectiveness as promising candidates for the management of oral Candida infections., Competing Interests: Conflict of interest The authors have no conflicts of interest to declare., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The essential oils of Thymus pallescens de Noé and Origanum floribundum Munby leaves and flowers were analyzed by CC and Cc/MS. The oil yields of T pallescens were 2.8% for the leaves and 3.7% for the flowers, while those of 0. floribundum were 2.0% for the leaves and 5.4% for the inflorescences. The major constituents of both the leaves and flowers of T pallescens were p-cymene (17.4% and 9.2%), 7-terpinene (10.6% and 14.1%) and carvacrol (39.0% and 48.3%), respectively, while those of O.floribundum were p-cymene (22.3% and 9.6%), y-terpinene (17.3% and 24.3%) and thymol (34.4% and 41.2%), respectively. For the both plants p-cyrnene and sesquiterpenes were essentially concentrated in leaves. [ABSTRACT FROM AUTHOR]