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Antibiotic residues in milk are of great public health concern since milk is being widely consumed by infants, youngster and adults throughout the globe. Therefore, eighty samples of different species including (cow, buffalo, camel and sheep) 20 of each type were collected from different markets and dairy shops located in Alexandria governorate, Egypt for determination antibiotic residues using HPLC. The obtained results were revealed that oxytetracycline, penicillin G and cloxacillin residues were detected at incidence rate (30, 30, 15, & 30%), (25, 20, 10, & 25%), and (10, 10, 0, &15%) with a mean values of (97.9±28.1, 85.5± 25.9, 48.4±28.0 & 122.9±29.2 ppb); (8.86 ± 3.60, 7.583± 4.73, 1.95± 0.95 &10.2 ± 3.40 ppb), and (39.3 ± 7.40, 21.5 ± 9.05, 0 & 37.6 ± 9.91 ppb) in examined cow, buffalo , camel and sheep's milk samples, respectively. Positive samples for antibiotic residues were compared with the maximum residual limits established by international standards. In addition, calculated daily intake for each antibiotic residues were compared with acceptable daily intake. One sample of camel and sheep milk may contain three antibiotic residues and these may cause public health hazards for consumers. Therefore, Legislations for monitoring and control antibiotics treatment of food producing animals should be adopted. [ABSTRACT FROM AUTHOR]