1. Evaluation of an in-clinic assay for the diagnosis of canine parvovirus.
- Author
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Decaro N, Desario C, Billi M, Lorusso E, Colaianni ML, Colao V, Elia G, Ventrella G, Kusi I, Bo S, and Buonavoglia C
- Subjects
- Albania, Animals, Colony Count, Microbial veterinary, DNA, Viral genetics, DNA, Viral metabolism, Diarrhea diagnosis, Diarrhea virology, Dog Diseases virology, Dogs, Feces virology, Hemagglutination Tests veterinary, Italy, Molecular Sequence Data, Parvoviridae Infections diagnosis, Parvoviridae Infections virology, Real-Time Polymerase Chain Reaction veterinary, Sensitivity and Specificity, Sequence Analysis, DNA veterinary, Spain, Colony Count, Microbial methods, Diarrhea veterinary, Dog Diseases diagnosis, Hemagglutination Tests methods, Parvoviridae Infections veterinary, Parvovirus, Canine isolation & purification, Real-Time Polymerase Chain Reaction methods
- Abstract
The results of a study designed to evaluate the performance of an in-clinic test for the detection of canine parvovirus (CPV) are reported. A total of 150 faecal samples collected from dogs with acute diarrhoea were tested using the in-clinic test, a haemagglutination assay (HA) and a real-time PCR assay for CPV detection, quantification and characterisation. CPV was detected in 66, 73, and 101 faecal samples by in-clinic, HA and PCR testing, respectively. The relative sensitivity and specificity of the in-clinic test were 86.3% and 96.1%, respectively, when the test was compared to HA, and 65.3% and 100%, respectively, when compared to real-time PCR. The sample distribution according to the virus type was CPV-2a, n=44; CPV-2b, n=11; CPV-2c, n=44, CPV-2, n=2, as determined by minor groove binder probe assays and/or sequence analysis. The percentage of positive in-clinic tests was 70.5% for CPV-2a, 72.7% for CPV-2b and 75.0% for CPV-2c (P>0.05). Using real-time PCR as the reference standard for CPV detection, the in-clinic test was more specific than HA and had comparable sensitivity to HA, demonstrating detection rates similar to those previously observed for other rapid in-clinic tests. The in-clinic test was also able to detect all CPV types at equivalent rates., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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