1. A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants.
- Author
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Jäckel S, Eiden M, Balkema-Buschmann A, Ziller M, van Vuren PJ, Paweska JT, and Groschup MH
- Subjects
- Africa South of the Sahara epidemiology, Animals, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay methods, Goat Diseases virology, Goats, Immunoglobulin G blood, Rift Valley Fever diagnosis, Rift Valley Fever immunology, Rift Valley Fever virology, Sheep, Sheep Diseases virology, Viral Proteins immunology, Viral Proteins metabolism, Yemen epidemiology, Enzyme-Linked Immunosorbent Assay veterinary, Glycoproteins metabolism, Goat Diseases diagnosis, Rift Valley Fever veterinary, Rift Valley fever virus immunology, Sheep Diseases diagnosis
- Abstract
Rift Valley fever virus (RVFV) is an emerging zoonotic pathogen that causes high morbidity and mortality in humans and livestock. In this paper, we describe the cloning, expression and purification of RVFV glycoprotein Gn and its application as a diagnostic antigen in an indirect ELISA for the specific detection of RVF IgG antibodies in sheep and goats. The performance of this Gn based ELISA is validated using a panel of almost 2000 field samples from sheep and goats from Mozambique, Senegal, Uganda and Yemen. All serum samples were also tested by virus neutralization test (VNT), the gold standard method for RVFV serological testing. Compared to the VNT results the Gn based ELISA proved to have an excellent sensitivity (94.56%) and specificity (95.57%). Apart from establishing this new diagnostic assay, these results also demonstrate a close correlation between the presence of RVFV Gn and neutralizing antibodies., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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