5 results on '"Zofou, Denis"'
Search Results
2. Anti-hyperglycaemic globulins from selected Cucurbitaceae seeds used as antidiabetic medicinal plants in Africa.
- Author
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Teugwa, Clautilde Mofor, Boudjeko, Thaddée, Tchinda, Bruno Tugnoua, Mejiato, Pascaline Chouadeu, and Zofou, Denis
- Subjects
PLANT proteins ,LIPID analysis ,PLANT protein analysis ,ANALYSIS of variance ,ANIMAL experimentation ,BLOOD sugar ,CONFIDENCE intervals ,DIABETES ,ELECTROPHORESIS ,GLOBULINS ,GLUCOSE tolerance tests ,MEDICINAL plants ,RATS ,SEEDS ,SOLID phase extraction ,ALBUMINS ,THERAPEUTICS - Abstract
Background: The recent epidemic of diabetes mellitus (DM) in Africa, coupled with rampant poverty, is an indication of the urgent need to develop new efficacious, cheaper and more available drugs to face this growing public health challenge. A number of plants products among which the protein-rich Cucurbitaceae seeds are commonly used in traditional medicine with increasing acclaimed efficacy against DM. The aim of this study was to analyse and evaluate the hypoglycaemic activity of storage proteins of five species of Cucurbitaceae, which include Telfairia occidentalis, Citrullus lanatus, Lagenaria siceraria, Cucumeropsis mannii and Cucurbita moschata. Methods: The different families of storage proteins were extracted following differential solubility, and their contents were estimated using the Bradford method. The analysis of these proteins was done by electrophoresis in non-denaturing and denaturing conditions. The evaluation of hypoglycaemic properties of various globulins extracted was performed on male Wistar rats by the oral glucose tolerance test. Results: The results showed that among the proteins extracted, globulins constitute the most abundant class of storage proteins in all five species selected. Citrullus lanatus and Cucurbita moschata presented the highest levels of globulin (275.34 and 295.11 mg/g dry matter, respectively). The results of electrophoresis showed that all species possess acidic and neutrals albumins and globulins, with molecular weight of protein subunits ranging from 6.36-44.11 kDa for albumins, 6.5-173.86 kDa for globulins and 6.5-49.66 kDa for glutelins. The 6.36 kDa of albumin subunit protein and the 6.5 kDa of globulin subunit protein were present in all the species. The oral glucose tolerance test showed that the globulins of the seeds of all species except Cucumeropsis mannii caused significant drop in blood sugar (88 - 137.80%, compared to the controls, p<0.05). Conclusions: These findings showed that the selected Cucurbitaceae seeds contained globulins with significant anti-hyperglycaemic activity. It is therefore highly encouraged to pursue investigations towards development of peptide-drugs and/or phytomedicines from these bioactive proteins which could be used as affordable alternative therapy against DM. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
3. The needs of biomedical science training in Africa: Perspectives from the experience of young scientists.
- Author
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Zofou, Denis, Abimbola, Seye, Norice, Carmelle T., Samje, Moses, Traore, Zoumana Isaac, Oyewale, A. Oyediran, Oraka, Chinedu, and Kadigi, Daudi Mussa
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MEDICAL sciences , *SURVEYS , *SCIENTISTS , *STREAMING media , *INFRASTRUCTURE (Economics) - Abstract
The article discusses the need of the needs of biomedical science training in Africa. To conduct the survey of the region, a total of 107 young scientists were recruited through existing international networks and interviewed via a web-based program, on the current status of biomedical research in their different institutions. Information on the obstacles for biomedical science research which include the lack of infrastructure and financial support from government is presented.
- Published
- 2011
4. Hypericum lanceolatum (Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark.
- Author
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Zofou, Denis, Kowa, Théodora K., Wabo, Hippolyte K., Ngemenya, Moses N., Tane, Pierre, and Titanji, Vincent P. K.
- Subjects
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MALARIA , *HYPERICUM , *ANTIMALARIALS , *PUBLIC health - Abstract
Background: Malaria is a major public health threat in Africa, and traditional medicine continues to play a key role in its control especially in rural areas. A bioassay-guided fractionation was carried out in order to evaluate the antimalarial potential and the safety of the methanol extract of the Hypericum lanceolatum stem bark. Methods: The anti-plasmodial activity was assayed by the lactate dehydrogenase method (pLDH) against the multidrug-resistant W2mef laboratory strain, and a field isolate (SHF4) of Plasmodium falciparum. Cytotoxicity tests were carried out using the LLC-MK2 monkey kidney epithelial cells. Results: Five compounds were isolated from the most active and least cytotoxic ethylacetate sub-extract: betulinic acid (HLT1), 2,2',5,6'-tetrahydroxybenzophenone (HLT2), 5-hydroxy-3-methoxyxanthone (HLT3), 3-hydroxy-5- methoxyxanthone (HLT4) and HLT0 (yet to be identified). Three of the tested compounds presented significant anti-plasmodial activities (with 50% inhibitory concentration, IC50 < 5 μM), with 5-hydroxy-3-methoxyxanthone exerting the highest activity, followed by HLT0 and betulinic acid. All the compounds with significant antiplasmodial activity were non-cytotoxic, except betulinic acid which showed a 50% cytotoxic concentration, CC50 of 25 μg/mL. Conclusions: These findings justify the use of H. lanceolatum stem bark as anti-malarial by traditional healers of Western Cameroon, and could constitute a good basis for further studies towards development of new drug candidates or phytomedicines for malaria. [ABSTRACT FROM AUTHOR]
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- 2011
- Full Text
- View/download PDF
5. AfroDb: a select highly potent and diverse natural product library from African medicinal plants.
- Author
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Ntie-Kang F, Zofou D, Babiaka SB, Meudom R, Scharfe M, Lifongo LL, Mbah JA, Mbaze LM, Sippl W, and Efange SM
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- Africa, Biological Products analysis, Computer-Aided Design, Databases, Factual, Plants, Medicinal chemistry
- Abstract
Computer-aided drug design (CADD) often involves virtual screening (VS) of large compound datasets and the availability of such is vital for drug discovery protocols. We assess the bioactivity and "drug-likeness" of a relatively small but structurally diverse dataset (containing >1,000 compounds) from African medicinal plants, which have been tested and proven a wide range of biological activities. The geographical regions of collection of the medicinal plants cover the entire continent of Africa, based on data from literature sources and information from traditional healers. For each isolated compound, the three dimensional (3D) structure has been used to calculate physico-chemical properties used in the prediction of oral bioavailability on the basis of Lipinski's "Rule of Five". A comparative analysis has been carried out with the "drug-like", "lead-like", and "fragment-like" subsets, as well as with the Dictionary of Natural Products. A diversity analysis has been carried out in comparison with the ChemBridge diverse database. Furthermore, descriptors related to absorption, distribution, metabolism, excretion and toxicity (ADMET) have been used to predict the pharmacokinetic profile of the compounds within the dataset. Our results prove that drug discovery, beginning with natural products from the African flora, could be highly promising. The 3D structures are available and could be useful for virtual screening and natural product lead generation programs.
- Published
- 2013
- Full Text
- View/download PDF
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