1. Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica).
- Author
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Park Y, Zhang Z, Laremore TN, Li B, Sim JS, Im AR, Ahn MY, Kim YS, and Linhardt RJ
- Subjects
- Africa, Animals, Carbohydrate Sequence, Gastropoda enzymology, Mannose chemistry, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase metabolism, Molecular Sequence Data, Ovum chemistry, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, beta-N-Acetylhexosaminidases metabolism, Gastropoda chemistry, Glycosaminoglycans chemistry, Monosaccharides chemistry, Polysaccharides analysis, Polysaccharides chemistry
- Abstract
Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide -->4)-alpha-D-GlcNpAc (1-->4)-alpha-L-IdoAp2S(1-->, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex(2-4)-HexNAc(2)), high mannose (Hex(5-9)-HexNAc(2)), and complex (Hex(3)-HexNAc(2-10)) types. None showed core fucosylation.
- Published
- 2008
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