1. Localization of immunogenic domains in the human immunodeficiency virus type 2 envelope.
- Author
-
Huang ML, Essex M, and Lee TH
- Subjects
- Africa, Western, Amino Acid Sequence, Base Sequence, Blotting, Western, Cloning, Molecular, Deltaretrovirus Infections immunology, Epitopes, HIV Seropositivity immunology, Humans, Membrane Glycoproteins immunology, Molecular Sequence Data, Polymerase Chain Reaction, United States, Gene Products, env immunology, HIV Antibodies immunology, HIV Antigens immunology, HIV-2 immunology, Viral Envelope Proteins immunology
- Abstract
Highly immunogenic domains have not yet been defined in the extracellular protein of the human immunodefiency virus type 2 (HIV-2) envelope. In this study, six contiguous segments covering the entire HIV-2ST envelope were amplified and cloned into a bacterial expression vector to localize the relative immunogenic reactivity of different regions of the molecule by Western blot (immunoblot) analysis. Our results demonstrate that the extracellular protein of the HIV-2 envelope contains highly immunogenic epitopes with potential value as type-specific markers for HIV-2 infection.
- Published
- 1991
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