1. Chikungunya virus isolated in Lakshadweep islands in the Indian Ocean: evidence of the Central/East African genotype.
- Author
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Paramasivan R, Philip Samuel P, Thenmozhi V, Rajendran R, Victor Jerald Leo S, Dhananjeyan KJ, Krishnamoorthi R, Arunachalam N, and Tyagi BK
- Subjects
- Africa, Central, Africa, Eastern, Alphavirus Infections epidemiology, Alphavirus Infections genetics, Antibodies, Viral blood, Cell Line, Chikungunya virus genetics, Disease Outbreaks, Fluorescent Antibody Technique, Indirect, Genotype, Humans, Indian Ocean, Molecular Sequence Data, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, Alphavirus Infections virology, Chikungunya virus isolation & purification
- Abstract
Virological investigation was carried out to determine the etiology of suspected Chikungunya fever among humans reported in the Lakshadweep islands in the Indian Ocean. Three out of 23 acute sera samples showed cytopathological changes in Vero cell lines. Further, indirect immunofluorescence antibody test and reverse transcriptase-polymerase chain reaction studies demonstrated the Chikungunya virus etiology during the episode. E1 gene sequence analysis has confirmed the involvement of the Central/East African genotype of the Chikungunya virus.
- Published
- 2009