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Abstract Background Diet analysis is essential to understanding the functional role of large bird species in food webs. Morphological analysis of regurgitated bird pellet contents is time intensive and may underestimate biodiversity. DNA metabarcoding has the ability to circumvent these issues, but has yet to be done. Methods We present a pilot study using DNA metabarcoding of MT-RNR1 and MT-CO1 markers to determine the species of origin and prey of 45 pellets collected in Qinghai and Gansu Provinces, China. Results We detected four raptor species [Eurasian Eagle Owl (Bubo bubo), Saker Falcon (Falco cherrug), Steppe Eagle (Aquila nipalensis), and Upland Buzzard (Buteo hemilasius)] and 11 unique prey species across 10 families and 4 classes. Mammals were the greatest detected prey class with Plateau Pika (Ochotona curzoniae) being the most frequent. Observed Shannon’s and Simpson’s diversity for Upland Buzzard were 1.089 and 0.479, respectively, while expected values were 1.312 ± 0.266 and 0.485 ± 0.086. For Eurasian Eagle Owl, observed values were 1.202 and 0.565, while expected values were 1.502 ± 0.340 and 0.580 ± 0.114. Interspecific dietary niche partitioning between the two species was not detected. Conclusions Our results demonstrate successful use of DNA metabarcoding for understanding diet via a novel noninvasive sample type to identify common and uncommon species. More work is needed to understand how raptor diets vary locally, and the mechanisms that enable exploitation of similar dietary resources. This approach has wide ranging applicability to other birds of prey, and demonstrates the power of using DNA metabarcoding to study species noninvasively.

Dramatic increases in populations of wild ungulates have brought a new ecological issue in the Qinling mountains. Information on species’ niche differentiation will contribute to a greater understanding of the mechanisms of coexistence, so as to ultimately benefit the conservation and management of ecological communities. In this study, camera trapping was used to investigate spatial and temporal activity patterns of sympatric wild ungulates in the Qinling Mountains of China, where top predators were virtually absent. We obtained 15,584 independent detections of seven wild ungulate species during 93,606 camera-trap days from April 2014 to October 2017. Results showed that (i) the capture rate differed significantly across species, with the capture rate of reeve muntjac being significantly higher than that of other species; (ii) the wild boar had a higher occupancy rates (ψ = 0.888) than other six ungulates, and distance to settlements had a negative relationship with wild boar (β = −0.24 ± 0.17); (iii) the forest musk deer and mainland serow had low spatial overlaps with other five wild ungulates, while spatial overlap indices of any two given pairs of wild ungulates were relatively high; (iv) all wild ungulates species (expect wild boar) were mainly active during crepuscular and diurnal periods, and showed bimodal activity peaks at around 05:00–07:00 and 17:00–19:00; and finally, (v) all wild ungulates showed moderate to high temporal overlaps. The results provided detailed information of the spatial and temporal ecology of wild ungulate communities in forest ecosystems of China, which also would be a guide to establish conservation priorities as well as efficient management programs.

Studying the spatial and temporal interactions between sympatric animal species is essential for understanding the mechanisms of interspecific coexistence. Both Asiatic black bears (Ursus thibetanus) and brown bears (Ursus arctos) inhabit northeastern China, but their spatial–temporal patterns and the mechanism of coexistence were unclear until now. Camera traps were set in Heilongjiang Taipinggou National Nature Reserve (TPGNR) from January 2017 to December 2017 to collect photos of the two sympatric bear species. The Pianka index, kernel density estimation, and the coefficient of overlap were used to analyze the spatial and temporal patterns of the two sympatric species. Our findings indicated that the spatial overlap between Asiatic black bears and brown bears was low, as Asiatic black bears occupied higher elevations than brown bears. The two species’ temporal activity patterns were similar at sites where only one species existed, yet they were different at the co–occurrence sites. Asiatic black bears and brown bears are competitors in this area, but they can coexist by changing their daily activity patterns. Compared to brown bears, Asiatic black bears behaved more diurnally. Our study revealed distinct spatial and temporal differentiation within the two species in TPGNR, which can reduce interspecific competition and facilitate coexistence between them.

Personal injury and property damage caused by wildlife can worsen the relationship between humans and wildlife. In recent years, conflicts between herders and Tibetan brown bears (Ursus arctos pruinosus) (human–bear conflicts; HBCs) on the Qinghai-Tibetan Plateau have increased dramatically, severely affecting community motivation for the conservation of brown bears and other species. Understanding the types, effectiveness, and flaws of current HBC mitigation measures is critical to develop effective strategies to alleviate HBC. From 2017 to 2019, we conducted a systematic field survey regarding HBCs on the Qinghai-Tibetan Plateau. In addition, we invited bear specialists and multiple interest groups to hold an HBC seminar and proposed some potential mitigation strategies. We surveyed 312 families via semi-structured interviews and documented 16 types of HBC mitigation measures. A total of 96% of respondents were using more than two mitigation measures simultaneously. The effectiveness evaluation of HBC mitigation measures showed that: (1) removing food from winter homes while herders were at their summer pastures and asking people to keep watch of winter homes were effective at protecting food and houses; (2) traditional grazing methods (human guarding of livestock all day) and solar soundboxes (attached to livestock) were effective at protecting free-range livestock; (3) solar street lights had a deterrent effect on brown bears and were effective in protecting livestock, houses, and people; and (4) due to the unstable power supply of photovoltaic cells and improper installation of ground wires, electric fences were not ideal in practice. Evaluation of the potential mitigation measures at the seminar showed that upgrading electric fence technology, expanding electric fence pilot areas, installing diversionary feeders, and introducing bear spray were the most optimal solutions. This study provides a scientific basis for creating human–bear coexistence plans on the Qinghai-Tibet Plateau.