1. Micromanipulation of mouse gametes with laser microbeam and optical tweezers.
- Author
-
Enginsu ME, Schütze K, Bellanca S, Pensis M, Campo R, Bassil S, Donnez J, and Gordts S
- Subjects
- Animals, Dissection, Female, Infertility, Male physiopathology, Infrared Rays, Male, Mice, Mice, Inbred Strains, Reference Values, Sperm Count, Ultraviolet Rays, Fertilization in Vitro, Lasers, Micromanipulation, Optics and Photonics instrumentation, Zona Pellucida radiation effects
- Abstract
Micromanipulation of mouse gametes with a commercially available compact laser microbeam system was studied. Both the normal in-vitro fertilization (IVF) group and the laser zona dissection (LZD) group were tested under normal (2 x 10(6) motile spermatozoa/ml) and low (500,000 motile spermatozoa/ml) insemination conditions. Subzonal insemination (SUZI) was also tried in a small group of gametes and the results were compared with those of the low insemination groups. Fertilization rates and blastocyst formation rates for the IVF and the LZD-treated groups were respectively 53 and 60% and 60 and 78%, which were not significantly different. However, under low insemination conditions, the results were significantly better in the LZD-treated group (58% fertilization rate and 83% blastocyst formation rate) compared to the results of the IVF group (33 and 48%) (P < 0.05). The SUZI-treated group showed the lowest fertilization rate (18%). No significant difference between the LZD and the IVF group was observed with respect to parthenogenetic activation. LZD has a beneficial effect on fertilization rates in cases of reduced sperm quality.
- Published
- 1995
- Full Text
- View/download PDF