1. Effects of Zinc, Mercury, or Lead on [ 3 H]MK-801 and [ 3 H]Fluorowillardiine Binding to Rat Synaptic Membranes.
- Author
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Berríos-Cartagena N, Rubio-Dávila MM, Rivera-Delgado I, Feliciano-Bonilla MM, De Cardona-Juliá EA, and Ortiz JG
- Subjects
- Alanine metabolism, Animals, Neuroprotective Agents metabolism, Rats, Synaptic Membranes drug effects, Alanine analogs & derivatives, Dizocilpine Maleate metabolism, Lead pharmacology, Mercury pharmacology, Pyrimidines metabolism, Synaptic Membranes metabolism, Zinc pharmacology
- Abstract
Glutamate (Glu) is considered the most important excitatory amino acid neurotransmitter in the mammalian Central Nervous System. Zinc (Zn) is co-released with Glu during synaptic transmission and interacts with Glutamate receptors and transporters. We performed binding experiments using [
3 H]MK-801 (NMDA), and [3 H]Fluorowillardine (AMPA) as ligands to study Zn-Glutamate interactions in rat cortical synaptic membranes. We also examined the effects of mercury and lead on NMDA or AMPA receptors. Zinc at 1 nM, significantly potentiates [3 H]MK-801 binding. Lead inhibits [3 H]MK-801 binding at micromolar concentrations. At millimolar concentrations, Hg also has a significant inhibitory effect. These effects are not reversed by Zn (1 nM). Zinc displaces the [3 H]FW binding curve to the right. Lead (nM) and Hg (μM) inhibit [3 H]FW binding. At certain concentrations, Zn reverses the effects of these metals on [3 H]FW binding. These specific interactions serve to clarify the role of Zn, Hg, and Pb in physiological and pathological conditions., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2021
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