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Start Over Author "Molina-Jouve Carole" Topic yarrowia lipolytica
10 results on '"Molina-Jouve Carole"'

1. ProBio3 project: how to achieve scientific and technological challenges to boost the sustainable microbial production of lipids as biojet fuel and chemical compounds

Author

Allouche Yohan, Cameleyre Xavier, Guillouet Stéphane, Hulin Sébastien, Thevenieau France, Akomia Laure, and Molina-Jouve Carole

Subjects
ProBio3, renewable resources, BiojetFuel, green chemistry, oleaginous microorganisms, lipids, Rhodotorula glutinis, Yarrowia lipolytica, Streptomyces lividans, Cupriavidus necator, Oils, fats, and waxes, TP670-699
Abstract

The deal of ProBio3 project is to develop the microbial conversion on specific fatty acids of carbon substrates from renewable resources and industrial by-products. The main application fields are BiojetFuels and green chemistry. The objectives focus on the identification of renewable feedstock suitable for microbial nutritional requirements, the development of an intensive bioprocess, the proof of feasibility at pilot scale (m3) with the evaluation of environmental, economic and societal impacts. During 8 years, 16 French partners (9 research units LISBP, MICALIS, IJPB, IGM, IMFT, SQPOV, TSE, TWB, IFPen, 4 industries Airbus, EADS, Sofiproteol, Tereos et 3 technical centres ITERG, CVG, CREOL) associate their interdisciplinary competences from Life Sciences to Process Engineering including Economic and Social Sciences. With Investissement d’Avenir financial support, the expected impacts are increases of fundamental knowledge of lipid metabolism in oleaginous microorganisms, development of high-throughput tools to fasten industrial engineering strains and fermentation strategies, new extraction processes involving green solvents and realistic scale up studies towards an industrial pilot; with undeniable innovative aspects, the deal is to get competitive assets for leading international positions on a new biological route.

Published
2013
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5. Characterization of mycelial transition of Yarrowia lipolytica during oxidative cultures: comparison of optical methods and their limitation, impact of morphology on rheological behavior

Author

Fillaudeau, Luc, Manon, Yannick, Kraiem, Hazar, Timoumi, Asma, Nguyen, Tien Cuong, Le, Tuan, Anne-Archard, Dominique, Bideaux, Carine, Gorret, Nathalie, Molina-Jouve, Carole, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts (FERMAT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), School of Biotechnology and Food Technology, Hanoi University of Science and Technology (HUST), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)

Subjects
bioprocédé, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, taille de la particule, Biotechnologies, diffraction laser light scattering, cytometry, rheometry, granulometry, morphology, filamentous microorganism, CLD, PSD, focus beam reflectance, microscopy, settling kinetics, rhéométrie, biomasse, yarrowia lipolytica
Abstract

In : 2nd Braunschweig International Symposium on Pharmaceutical Engineering Research SPhERe, TU Braunschweig, Germany September 06 to 08, 2017.In : 2nd Braunschweig International Symposium on Pharmaceutical Engineering Research SPhERe, TU Braunschweig, Germany September 06 to 08, 2017.; Characterization of mycelial transition of Yarrowia lipolytica during oxidative cultures: comparison of optical methods and their limitation, impact of morphology on rheological behavior. 2. Braunschweig International Symposium on Pharmaceutical Engineering Research SPhERe

Published
2017

6. How to manage carbohydrate storage in oleaginous yeasts to optimize lipid accumulation under nitrogen or phosphorus limitations ?

Author

Cescut, Julien, Portelli, Bérangère, Molina-Jouve, Carole, Fillaudeau, Luc, Uribelarrea, Jean-Louis, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
lipids, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, biofuels, fermentation, yeast, lipid, rhodotorula glutinis, Biotechnologies, production microbienne, yarrowia lipolytica, métabolisme, lipide
Abstract

In : ECCE10+ECAB3+EPIC5 (10th European Congress of Chemical Engineering; 3rd European Congress of Applied Biotechnology and 5th European Process Intensification Conference), Topic : Biochemical Engineering / ECAB3 / Biocatalysis and biotransformation. Submission-1659, Nice, France, September 27th - October 1st 2015In : ECCE10+ECAB3+EPIC5 (10th European Congress of Chemical Engineering; 3rd European Congress of Applied Biotechnology and 5th European Process Intensification Conference), Topic : Biochemical Engineering / ECAB3 / Biocatalysis and biotransformation. Submission-1659, Nice, France, September 27th - October 1st 2015; How to manage carbohydrate storage in oleaginous yeasts to optimize lipid accumulation under nitrogen or phosphorus limitations ?. 10. European Congress of Chemical Engineering - ECCE 2015

Published
2015

7. Morphology and rheological behaviour of Yarrowia lipolitica during production of intra-cellular energetic molecules: Impact of lipid accumulation and genetic modifications

Author

Fillaudeau, Luc, Cescut, Julien, Anne-Archard, Dominique, Nicaud, Jean-Marc, Uribelarrea, Jean-Louis, Molina-Jouve, Carole, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut de Mécanique des Fluides, Université Fédérale Toulouse Midi-Pyrénées, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de mécanique des fluides de Toulouse (IMFT), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), and Université de Toulouse

Subjects
Yarrowia lipolytica, culture de levures, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, yeast culture, genetically engineered strain, intra-cellular, Biotechnologies
Abstract

In: Proceeding of 8th World Congress of Chemical Engineering, August 23-29, Montréal, Quebec, Canada. Eds. Canadian Society for Chemical Engineering. (Ontario, Canada), ISBN: 0-9208044-44-6, Session: Bioproducts, Bioreactors and Bioprocesses, L.0122, 2009In: Proceeding of 8th World Congress of Chemical Engineering, August 23-29, Montréal, Quebec, Canada. Eds. Canadian Society for Chemical Engineering. (Ontario, Canada), ISBN: 0-9208044-44-6, Session: Bioproducts, Bioreactors and Bioprocesses, L.0122, 2009; Efficient microbial conversion of carbohydrates from plant biomass into energetic molecules such as specific lipids is a real challenge in bioprocess. Our study focussed on the evolution of yeast morphology and rheological behaviour of cell broth during production of intra-cellular lipids. Three Yarrowia lipolytica strains (wild and genetically engineered strains) were investigated under controlled fed-batch culture including 3 steps (growth, nitrogen limitation and lipid accumulation). Physical (microscopy, rheology, particle size distribution, concentration) and biological (cell dried matter, lipid content) parameters were simultaneously characterised or quantified. Culture strategy enabled to reach up to 111gCDW/L in biomass and 0.36g/gCDW lipid. Results demonstrate that yeast strains are characterised by different morphologies. Lipid synthesis is a complex and vital metabolism which affects morphology and physical properties. Broth rheology depends on biomass concentration, lipid accumulation and morphology and affects bioprocess performances.

Published
2009

8. Modulation of the Glycerol Phosphate availability led to concomitant reduction in the citric acid excretion and increase in lipid content and yield in Yarrowia lipolytica.

Author

Sagnak, Rana, Cochot, Sandrine, Molina-Jouve, Carole, Nicaud, Jean-Marc, and Guillouet, Stéphane E.

Subjects
*DIPODASCACEAE, *GLYCERIN, *CITRIC acid, *ACYLTRANSFERASES, *LIPIDS
Abstract

In order to improve TriAcylGycerol (TAG) lipids accumulation in the yeast Yarrowia lipolytica on glucose, double over-expression of the major acyl-CoA:diacylglycerol acyltransferase encoding gene (ylDGA2) and of the glycerol-phosphate dehydrogenase encoding gene (ylGPD1) was carried out. The genes were over-expressed in a strain impaired for the mobilization of the accumulated lipids, through the deletion of the genes encoding acyl-coenzyme A oxidases (POX1-6 genes) and the deletion of the very efficient lipase attached to the lipid bodies, encoded by ylTGL4. This metabolic engineering strategy had the objective of pulling the C-flow into the TAG synthesis by increasing the availability of glycerol-3-phosphate and its binding to fatty acids for the TAG synthesis. This strain showed a strong improvement in production performances on glucose in terms of lipid content (increase from 18 to 55%), lipid yield (increase from 0,035 to 0.14 g g −1 ) and by-product formation (decrease in citric acid yield from 0.68 to 0.4 g g −1 ). For developing bioprocess for the production of triacylglycerol from renewable carbon sources as glucose it is of first importance to control the C/N ratio in order to avoid citric acid excretion during lipid accumulation. Our engineered strain showed a delay in the onset of citric acid excretion as suggested by the 15% modulation of the critical C/N ratio. [ABSTRACT FROM AUTHOR]

Published
2018
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9. Dynamic metabolic modeling of lipid accumulation and citric acid production by Yarrowia lipolytica.

Author

Robles-Rodriguez, Carlos Eduardo, Bideaux, Carine, Guillouet, Stéphane E., Gorret, Nathalie, Cescut, Julien, Uribelarrea, Jean-Louis, Molina-Jouve, Carole, Roux, Gilles, and Aceves-Lara, César Arturo

Subjects
*LIPID metabolism, *CITRIC acid, *DYNAMIC models, *MATHEMATICAL optimization, *MATHEMATICAL models, *COMPUTER simulation
Abstract

Yarrowia lipolytica has the capacity to accumulate large amounts of lipids triggered by a depletion of nitrogen in excess of carbon source. However, under similar conditions this yeast also produces citric acid decreasing the lipid conversion yield. Three dynamic metabolic models are presented to describe lipid accumulation and citric acid production by Yarrowia lipolytica . First and second models were respectively based on the Hybrid Cybernetic Modeling (HCM) and the Macroscopic Bioreaction Modeling (MBM) approaches. The third model was a new approach based on the coupling between MBM and fuzzy sets. Simulation results of the three models fitted acceptably the experimental data sets for calibration and validation. However, MBM is time-dependent to consider metabolic shifts, and thus impractical for further applications. HCM and Fuzzy MBM adequately managed and described metabolic shifts presenting highlighting features for control and optimization. HCM and Fuzzy MBM were statistically compared reflecting similar results. [ABSTRACT FROM AUTHOR]

Published
2017
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10. Yarrowia lipolytica as a model for bio-oil production

Author

Beopoulos, Athanasios, Cescut, Julien, Haddouche, Ramdane, Uribelarrea, Jean-Louis, Molina-Jouve, Carole, and Nicaud, Jean-Marc

Subjects
*YEAST fungi biotechnology, *BIOMASS energy, *HYDROPHOBIC surfaces, *LIPID metabolism, *ARACHIDONIC acid, *YEAST fungi genetics, *BIOCONVERSION, *FATTY acids
Abstract

Abstract: The yeast Yarrowia lipolytica has developed very efficient mechanisms for breaking down and using hydrophobic substrates. It is considered an oleaginous yeast, based on its ability to accumulate large amounts of lipids. Completion of the sequencing of the Y. lipolytica genome and the existence of suitable tools for genetic manipulation have made it possible to use the metabolic function of this species for biotechnological applications. In this review, we describe the coordinated pathways of lipid metabolism, storage and mobilization in this yeast, focusing in particular on the roles and regulation of the various enzymes and organelles involved in these processes. The physiological responses of Y. lipolytica to hydrophobic substrates include surface-mediated and direct interfacial transport processes, the production of biosurfactants, hydrophobization of the cytoplasmic membrane and the formation of protrusions. We also discuss culture conditions, including the mode of culture control and the culture medium, as these conditions can be modified to enhance the accumulation of lipids with a specific composition and to identify links between various biological processes occurring in the cells of this yeast. Examples are presented demonstrating the potential use of Y. lipolytica in fatty-acid bioconversion, substrate valorization and single-cell oil production. Finally, this review also discusses recent progress in our understanding of the metabolic fate of hydrophobic compounds within the cell: their terminal oxidation, further degradation or accumulation in the form of intracellular lipid bodies. [Copyright &y& Elsevier]

Published
2009
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