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Along with the cellulase enzyme, xylanase plays an efficient role in the production of biofuel from agricultural wastes by degrading the xylan sugar present in the hemicellulose of cell wall. This study aims to improve the sugar production from biomass by the use of different enzymes with surfactant. The objective of this study is to compare sugar production and bioethanol production from sodium hydroxide along polyethylene glycol pre-treated paddy straw (Oryza sativa L.) with different combination of xylanase and cellulose enzymes along with lignin-degrading laccase enzyme. In results, 10.87 g/l of ethanol with saccharification of 64.51% ± 0.90 was obtained when xylanase and laccase were used, while 18.40 ± 0.56 g/l of ethanol with saccharification of 84.01%±1.09 was obtained when cellulase and laccase enzymes were used. Maximum bioethanol production was found to be 19.20 ± 0.26g/l, which was obtained by combination of xylanase, cellulase and laccase enzymes together at 37 °C after 36 h. [ABSTRACT FROM AUTHOR]