1. Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice.
- Author
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Zhang H, Wang M, Zhou Y, Bao S, Wang F, and Li C
- Subjects
- Animals, Humans, Mice, Cell Line, Tumor, Male, Reactive Oxygen Species metabolism, Antioxidants pharmacology, NF-kappa B metabolism, Colitis chemically induced, Colitis drug therapy, Disease Models, Animal, Xanthophylls pharmacology, Oxidative Stress drug effects, Azoxymethane toxicity, Dextran Sulfate, Tumor Necrosis Factor-alpha metabolism, Mice, Inbred C57BL, Colitis-Associated Neoplasms drug therapy, Colitis-Associated Neoplasms prevention & control, Colitis-Associated Neoplasms pathology
- Abstract
In this study, we investigated the protective effects of astaxanthin (AST) against oxidative stress induced by the combination of azoxymethane (AOM) and dextran sulfate sodium (DSS) in colitis-associated cancer (CAC) and TNF-α-induced human colorectal cancer cells (SW480), as well as the underlying mechanism. In vitro experiments revealed that astaxanthin reduced reactive oxygen species (ROS) generation and inhibited the expression of Phosphorylated JNK (P-JNK), Phosphorylated ERK (P-ERK), Phosphorylated p65 (P-p65), and the NF-κB downstream protein cyclooxygenase-2 (COX-2). In vivo experiments showed that astaxanthin ameliorated AOM/DSS-induced weight loss, shortened the colon length, and caused histomorphological changes. In addition, astaxanthin suppressed cellular inflammation by modulating the MAPK and NF-κB pathways and inhibiting the expression of the proinflammatory cytokines IL-6, IL-1β, and TNF-α. In conclusion, astaxanthin attenuates cellular inflammation and CAC through its antioxidant effects. more...
- Published
- 2024
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