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Your search keyword '"Danesh-Azari, Hamid-Reza"' showing total 1 results
Start Over Author "Danesh-Azari, Hamid-Reza" Topic x-ray diffraction
1 results on '"Danesh-Azari, Hamid-Reza"'

1. Structure-Guided Redesign of CYP153A M.aq for the Improved Terminal Hydroxylation of Fatty Acids.

Author

Hoffmann, Sara M., Danesh ‐ Azari, Hamid ‐ Reza, Spandolf, Claudia, WeissENborn, Martin J., Grogan, Gideon, and Hauer, Bernhard

Subjects
*CYTOCHROME P-450, *MOLECULAR structure, *FATTY acids, *HYDROXYLATION, *CARBOXYL group, *MOLECULAR recognition
Abstract

The structure of a P450 ω-hydroxylase bound to its fatty acid product was determined, which revealed a narrow substrate tunnel that leads to the heme. The introduction of an arginine side chain in proximity to the carboxyl group of the fatty acid led to a reduced KM value for dodecanoic acid, which suggests the importance of an anchoring point in the active site. An increase in the flexibility of the substrate recognition region was also engineered, which resulted in a threefold improved product formation. [ABSTRACT FROM AUTHOR]

Published
2016
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