1. Biomimicry of oil infused layer on 3D printed poly(dimethylsiloxane): Non-fouling, antibacterial and promoting infected wound healing.
- Author
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Shi G, Wang Y, Derakhshanfar S, Xu K, Zhong W, Luo G, Liu T, Wang Y, Wu J, and Xing M
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Bacterial Adhesion drug effects, Cell Proliferation drug effects, Cell Survival drug effects, Disease Models, Animal, Escherichia coli drug effects, Fibroblasts cytology, Fibroblasts drug effects, Granulation Tissue drug effects, Granulation Tissue pathology, Ions, Mice, Inbred BALB C, Microbial Sensitivity Tests, Nanocomposites chemistry, Nanocomposites ultrastructure, Rheology, Silver chemistry, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus drug effects, Temperature, Wound Infection pathology, Anti-Bacterial Agents therapeutic use, Biofouling, Biomimetics, Dimethylpolysiloxanes pharmacology, Oils chemistry, Printing, Three-Dimensional, Wound Healing drug effects, Wound Infection drug therapy
- Abstract
The nepenthes-inspired slippery liquid-infused surface has led to multiple potentials in biomedical devices' design. This study aims to develop a biomimetic, environmentally-friendly slippery layer of oil-infused 3D printed polydimethylsiloxane with anti-bacterial nanosilver (iPDMS/AgNPs) for wound dressing. The engineered 3D printed iPDMS can cater the different requirements of wounds with antifouling, anti-blood staining, and kill bacteria. iPDMS/AgNPs not only exhibits biocompatibility, against adherence and effective antibacterial activity but also effectively promotes neo-epithelial and granulation tissue formation to accelerate wound healing in vivo. Optimized rheologic parameters were obtained for the 3D printable iPDMS pre-polymerization condition. Scanning electronic micrograph (SEM) and Energy Dispersive Spectrometer (EDS) show a uniform mesh with AgNPs dotted on the printed dressing. No cytotoxicity of iPDMS/AgNPs has been found via cell Counting Kit-8(CCK-8) assay. Meanwhile, the membranes infused with silicon oil effectively prevented from the adherence of the two standard drug-resistant bacteria, Staphylococcus aureus and Escherichia coli (PDMS vs. PDMS+oil, p < 0.05; PDMS+0.5%AgNPs vs. iPDMS+0.5%AgNPs, p < 0.05; PDMS+2.5%AgNPs vs. iPDMS+2.5%AgNPs, p < 0.05). By bacteria co-culture model iPDMS/AgNPs can kill about 80% of Staphylococcus aureus and Escherichia coli. When applied to a full-thickness wound defect model of murine, iPDMS/AgNPs was effective in anti-infection. It also promotes the epithelialization, the granulation tissue formation, and wound healing. These findings demonstrate that iPDMS/AgNPs may have therapeutic promise as an ideal wound dressing shortly., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2019
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