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Your search keyword '"Collagen Type III radiation effects"' showing total 2 results
Start Over Descriptor "Collagen Type III radiation effects" Topic wound healing
2 results on '"Collagen Type III radiation effects"'

1. Differing energy densities with laser 670nm InGaP controls inflammation and collagen reorganization in burns.

Author

Maligieri LAO, Neves LMG, de Morais DT, Domingues RF, de Aro AA, Pimentel ER, do Amaral MEC, Esquisatto MAM, Dos Santos GMT, and Mendonça FAS

Subjects
Animals, Blotting, Western, Burns immunology, Burns metabolism, Burns pathology, Collagen Type I metabolism, Collagen Type I radiation effects, Collagen Type III metabolism, Collagen Type III radiation effects, Dose-Response Relationship, Radiation, Fibroblasts radiation effects, Gallium, Glycosaminoglycans metabolism, Glycosaminoglycans radiation effects, Hydroxyproline metabolism, Hydroxyproline radiation effects, Indium, Inflammation, Male, Matrix Metalloproteinase 9 metabolism, Matrix Metalloproteinase 9 radiation effects, Phosphines, Rats, Rats, Wistar, Skin immunology, Skin metabolism, Skin pathology, Transforming Growth Factor beta1 immunology, Transforming Growth Factor beta1 radiation effects, Vascular Endothelial Growth Factor A metabolism, Vascular Endothelial Growth Factor A radiation effects, Burns radiotherapy, Low-Level Light Therapy methods, Skin radiation effects, Wound Healing radiation effects
Abstract

Purpose: This study compared different energy densities of laser on second degrees burns in rats aiming to determine the most effective dosimetry in stimulation of the healing process., Methods: Burns were induced in the dorsal skin of 54 animals divided into three groups (n: 18): 1-without treatment; 2-irradiated lesions by the Indium Gallium Phosphide (InGaP) 670nm (4.93J/cm 2 ) laser; 3-irradiated lesions by the InGaP-670nm (9.86J/cm 2 ) laser. Samples were collected on the 2, 10 and 18 days after injury for structural, morphometry, biochemical analysis and Western blotting., Results: The energy densities examined were effective in significantly increasing the total number of fibroblasts and blood vessels and reduce the number of inflammatory cells particularly in irradiated lesions with 9.86J/cm 2 . This same energy density significantly increased the amount of GAGs (Glycosaminoglycans), decreased the TGF-β1 (Transforming Growth Factor β1) and increased the VEGF (Vascular and Endothelial Growth Factor) during the experimental period. This energy density also significantly increased the Collagen type I and decreased Collagen type III and the active isoform of metalloproteinase 9 (MMP-9)., Conclusions: The energy density of 9.86J/cm 2 was more effective in promoting cellular responses related to neoangiogenesis, decreasing inflammation and collagen fibers reorganization., (Copyright © 2017 Elsevier Ltd and ISBI. All rights reserved.) more...

Published
2017
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2. Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats.

Author

Gonçalves RV, Mezêncio JM, Benevides GP, Matta SL, Neves CA, Sarandy MM, and Vilela EF

Subjects
Animals, Low-Level Light Therapy methods, Male, Rats, Rats, Wistar, Collagen Type I radiation effects, Collagen Type III radiation effects, Lasers, Semiconductor therapeutic use, Skin radiation effects, Wound Healing radiation effects
Abstract

This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals' backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm(2)); group 2 (L30): GaAlAs laser (30 J/cm(2)); group 3 (L60): GaAlAs laser (60 J/cm(2)); group 4 (D): Dersani ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 + or - 7.10%), which differed from control (29.86 + or - 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 + or - 2.23%) and group 2 (L30; 2.87 + or - 2.40%) differed significantly from control (1.77 + or - 2.97%) on the 20th day of the experiment. more...

Published
2010
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