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Background/aim: Bacterial adherence to a suture material is one of the main causes of surgical site infections. An antibacterial suture material with enhanced wound healing function may protect the surgical site from infections. Thus, the present study aimed to investigate the synergistic effect of propolis and biogenic metallic nanoparticles when combined with silk sutures for biomedical use., Materials and Methods: Silver nanoparticle (AgNP) synthesis was carried out via a microbial-mediated biological route and impregnated on propolis-loaded silk sutures using an in situ process. Silk sutures fabricated with propolis and biosynthesized AgNPs (bioAgNP-propolis-coated sutures) were intensively characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The antibacterial characteristics of the bioAgNP-propolis-coated sutures were evaluated using the agar plate method. The biocompatibility of the bioAgNP-propolis- coated sutures was evaluated using 3T3 fibroblast cells, and their wound-healing potential was also investigated., Results: BioAgNP-propolis-coated sutures displayed potent antibacterial activity against pathogenic gram-negative and gram-positive bacteria, Escherichia coli and Staphylococcus aureus, respectively. BioAgNP-propolis-coated silk sutures were found to be biocompatible with 3T3 fibroblast cell culture. In vitro wound healing scratch assay also demonstrated that the extract of bioAgNP-propolis-coated sutures stimulated the 3T3 fibroblasts’ cell proliferation., Conclusion: Coating the silk sutures with propolis and biogenic AgNPs gave an effective antibacterial capacity to surgical sutures besides providing biocompatibility and wound healing activity., Competing Interests: None declared., (This work is licensed under a Creative Commons Attribution 4.0 International License.)

Boron containing compounds (BCCs) have recently been used for pharmaceutical applications. Zinc, an essential element, is known to be one of the most promising biodegradable metals. The present study was conducted to determine the wound healing properties of zinc borate with its antimicrobial, antifibrinolytic and enzyme inhibitory characteristics. In vitro scratch wound healing assay revealed that zinc borate at 0.01 µg/mL concentration stimulated the proliferation of 3T3 fibroblast cells after 24 h of scar formation. The highest enzyme inhibition was observed against collagenase at 1 mg/mL (81.5%). Minimum inhibition concentration (MIC) values were determined as 1 mg/mL and 0.5 mg/mL against Candida albicans and Staphylococcus aureus, respectively. Zinc borate did not have antifibrinolytic activity at 1, 0.5 and 0.1 mg/mL concentrations. It can be suggested that zinc borate can be used effectively to improve the wound healing process and to prevent the possible wound infections. [ABSTRACT FROM AUTHOR]

Copyright of Mugla Journal of Science & Technology is the property of Mugla Journal of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The genus Hypericum sp. has a number of uses in traditional medicine like curing the burns, ulcers, haemorrhoids and wound healing. The species Hypericum lydium Boiss. (Hypericaceae), however, has not been known to have any properties related to the healing of injuries or antimicrobial working against the oral microorganisms. The present study was aimed to evaluate the efficiency of H. lydium in soft tissue healing and its capacity to prevent infections after dental extraction. H. lydium was extracted with ethanol and the obtained extract was tested for its inhibition ability on extracellular matrix-degrading enzymes; collagenase, hyaluronidase and elastase. To determine the cytotoxicity and wound healing capacity of the extract, MTT and in vitro scratch wound healing assay were performed using the NIH-3T3 fibroblast cells, respectively. Antimicrobial activity was investigated by microdilution method against oral pathogenic microorganisms. The highest enzyme inhibition activity was determined against elastase (80.27±0.1%). According to the cytotoxic activity results, the IC50 value of the H. lydium was found to be 82.20±4.05 µg/mL. Scratch wound healing assay of the extract exhibited a significant enhancement at 24 h with a closed wound area when compared with the control. The extract showed potent antimicrobial properties against oral pathogenic microorganisms. The results of the study revealed out that H. lydium can be considered as a natural compound for dental industry to improve soft tissue healing and to prevent the possible infections after dental extraction. [ABSTRACT FROM AUTHOR]

Copyright of Mugla Journal of Science & Technology is the property of Mugla Journal of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background/aim: The potential inhibitory effects of Ankaferd Blood Stopper (ABS) against biofilm formation of oral microorganisms and its capacity for collagenase, hyaluronidase, and elastase inhibitions that have important roles in wound healing have been investigated. Materials and methods: The wound healing potential was determined by its inhibition ability on collagenase, hyaluronidase, and elastase enzyme activities and was evaluated via scratch wound healing assay on murine 3T3 fibroblasts. The antibiofilm activity was tested against eight oral microorganisms using the crystal violet staining method. Results: At 10% ABS successfully inhibited the biofilm formation of the tested microorganisms. Enzyme inhibition analysis revealed that 3% ABS significantly inhibited all three enzymes related to wound healing. The scratch assay showed that wound closure was faster than that of the control for the 3% ABS/plate. Conclusion: The findings of the present study indicated that ABS has effective wound healing potential with its strong antibiofilm activity against oral cavity microorganisms. [ABSTRACT FROM AUTHOR]