Aurélie Metay, Guillaume Fried, Elena Kazakou, Marie-Charlotte Bopp, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Agrosystèmes Biodiversifiés (UMR ABSys), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Montpellier (CIHEAM-IAMM), Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Unité entomologie et plantes invasives (LSV Montpellier), Laboratoire de la santé des végétaux (LSV), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and This research was supported by Occitanie Region (Arrete modificatif N. 19008795/ALDOCT-000660 Subvention d'investissement, Allocations de recherche doctorales 2019) and the Office Francais de la Biodiversite (ECOPHYTO II: Axe 2 -Action 8 and 9, N.SIREPA: 4148) as part of the SAVING project: Spatio-temporal dynamics of weed species communities in response to soil management practices in vineyards and consequences for grapevines: transition to zero glyphosate management. We would like to thank all winegrowers who provided management information and access to their farms. Thanks to the Biovigilance Flore network including all the people from SRAL and FREDON who performed the surveys, Nicolas Andre (FREDON Occitanie), Jacques Grosman (DGAL, SRAL Rhone-Alpes), and Olivier Pillon (SRAL Champagne) for data management at the regional level, and the Ministry of Agriculture for funding the monitoring. Warm thanks to Margot Puiraveau who gathered the dataset in 2015. The study utilised data provided through the TRY initiative on plant traits (http://www.try-db.org). The TRY initiative and database is hosted, developed and maintained by J. Kattge and G. Beonisch (Max Planck Institute for Biogeochemistry, Jena, Germany). TRY is currently supported by DIVERSITAS/Future Earth and the German Centre for Integrative Biodiversity Research (iDiv) HalleJena-Leipzig. The authors have no conflicts of interest to declare.
International audience; Highlights:• Region (53%) and season (28%) explained most of Community Weighted Means variance.• Weed management practices explained 19% of Community Weighted Means variance.• Chemically weeded communities showed trait values of ruderal strategies.• Mowed plots were associated with more competitive communities.• Tillage favoured weed communities with higher lateral spread ability.Abstract:Winegrowers have diversified their weed management practices over the last two decades changing the structure and the composition of weed communities. Complementary to taxonomic studies, trait-based approaches are promising ways for a better understanding of weed community responses to environmental and agronomic filters. In the present study, the impacts of climate, soil characteristics, seasons and weed management practices (chemical weeding, tillage and mowing) were assessed on weed communities from 46 plots in three French wine growing regions (Champagne, Languedoc and Rhone valley). These agro-environmental gradients structuring weed communities according to their combinations of traits were highlighted using multivariate analysis (RLQ). The impacts of these filters on Community Weighted Means (CWM) and Community Weighted Variance (CWV) of weed communities were analysed using mixed and null modelling. Our results showed that spatio-temporal and weed management practice variables explained from 13% to 48% of the total variance of CWM (specific leaf area, maximum height, seed mass, flowering onset and duration and lateral spread). Region, seasonality and management practices explained 53%, 28% and 19% of CWM marginal variance, respectively. Weed management impacted CWM and CWV through two main gradients: (i) a soil disturbance gradient with high mechanical disturbance of soil in tilled plots and low mechanical disturbance in chemically weeded plots and (ii) a vegetation cover gradient with high vegetation abundance in mowed plots compared to barer soils in tilled and chemically weeded plots. In Languedoc, chemical weeding filtered weed communities with ruderal strategy trait values (low seed mass, small-stature) while mowed communities were more competitive (higher seed mass, higher stature and lower SLA). In Languedoc and Champagne, tillage favoured communities with high seed mass that increases the viability of buried seeds and high lateral spread values associated to the ability to resprout after tillage. This study demonstrated that trait-based approaches can be successfully applied to perennial cropping systems such as vineyards, in order to understand community assembly to better guide weed management practices.