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Globally, river pesticide concentrations are associated with regional and local stream invertebrate diversity declines. Pesticides often co-occur with elevated nutrients (e.g. nitrogen and phosphorus) and sediments related to agriculture, making their individual effects difficult to disentangle. These effects are also less well studied in Asia, than in other geographic regions. Within Asia, India is one of the largest producers and users of pesticides and has approximately 60% of total land mass used for agriculture. Here we examine the responses of Indian river invertebrate communities subjected to malathion, nutrients, and sediment additions in a semi-orthogonal design, in three sequential (through time) short-term (120 h) mesocosm experiments. Additionally, a series of single-species toxicity tests were run that used 24 h exposure and 72 h recovery to examine the sensitivity of 13 local invertebrate taxa to malathion, and 9 taxa to cypermethrin, comparing these results to those from other biogeographic regions. Mesocosm results indicate that malathion exposure had a major effect compared to other stressors on communities, with a lesser effect of nutrients and/or sediments. In mesocosms, taxa richness, total abundance and the abundance of sensitive species all declined associated with malathion concentrations. Comparisons of organism sensitivities from other geographic locations and those in the current paper suggest taxa in India are relatively tolerant to malathion and cypermethrin. Our results further reinforce that the high observed aquatic pesticide concentrations known to occur in Asian freshwater ecosystems are likely to be negatively affecting biodiversity, homogenising biota towards those most stress tolerant., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Concentrations of major ions in coal mine discharge waters and unconventional hydrocarbon produced waters derived from coal bed methane (CBM) production, are potentially harmful to freshwater ecosystems. Bicarbonate is a major constituent of produced waters from CBM and coal mining. However, little is known about the relative toxicity of differing ionic proportions, especially bicarbonate, found in these CBM waters. As all freshwater invertebrates tested are more acutely sensitive to sodium bicarbonate (NaHCO 3 ) than sodium chloride (NaCl) or synthetic sea water, we tested the hypotheses that toxicity of CBM waters are driven by bicarbonate concentration, and waters containing a higher proportion of bicarbonate are more toxic to freshwater invertebrates than those with less bicarbonate. We compared the acute (96 h) lethal toxicity to six freshwater invertebrate species of NaHCO 3 and two synthetic CBM waters, with ionic proportions representative of water from CBM wells across New South Wales (NSW) and Queensland (Qld), in Australia. The ranking of LC50 values expressed as total salinity was consistent with the hypotheses. However, when toxicity was expressed as bicarbonate concentration, the hypothesis that the toxicity of coal bed waters would be explained by bicarbonate concentration was not well supported, and other ionic components were either ameliorating or exacerbating the NaHCO 3 toxicity. Our findings showed NaHCO 3 was more toxic than NaCl and that the NaHCO 3 proportion of synthetic CBM waters drives toxicity, however other ions are altering the toxicity of bicarbonate., (© 2022. The Author(s).)

Freshwater biota are at risk globally from increasing salinity, including increases from deicing salts in cold regions. A variety of metrics of toxicity are used when estimating the toxicity of substances and comparing the toxicity between substances. However, the implications of using different metrics are not widely appreciated. Using the mayfly Colobruscoides giganteus (Ephemeroptera: Colobruscoidea), we compare the toxicity of seven different salts where toxicity was estimated using two metrics: (1) the no-effect concentrations (NEC) and (2) the lethal concentrations for 10, 25 and 50% of the test populations (LC x ). The LC x values were estimated using two different models, the classic log-logistic model and the newer toxicokinetic-toxicodynamic (TKTD) model. The NEC and both types of LC x values were estimated using Bayesian statistics. We also compared the toxicity of two salts (NaCl and CaCl 2 ) for C. giganteus at water temperatures of 4 °C, 7 °C and 15 °C using the same metrics of toxicity. Our motivation for using a mayfly to assess salinity toxicity was because mayflies are generally salt sensitive, are ecologically important and are common in Australian (sub-)alpine streams. The temperature ranges were chosen to mimic winter, spring and summer water temperatures for Australian (sub-)alpine streams. Considering 144-h classical LC x values, we found toxicity differed between various salts, i.e., the lowest 144-h LC 50 (8 mS/cm) for a salt used by a ski resort was half that of the highest 144-h LC 50 from artificial marine salts and CaCl 2 applied to roads (16 mS/cm). The analytical grade NaCl (as shown by 144-h LC 50 value at 7 °C) was substantially more toxic (7.3 mS/cm) compared to analytical grade CaCl 2 (12.5 mS/cm). Yet for NEC values, there were comparably fewer differences in toxicity between salts and none between the same salts at different temperatures. We conclude that LC x values are better suited to compare the difference in toxicity between substances or between the same substance at different test temperatures, while NEC values are better suited to estimating concentrations of substances that have no effect to the test species and endpoint measured under laboratory conditions., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Within human modified ecosystems the effects of individual stressors are difficult to establish amid co-occurring biological processes, environmental gradients and other stressors. Coupled examination of several endpoints across different levels of organisation may help elucidate the individual and combined effects of stressors and interactions. Malathion is a commonly used organophosphate pesticide that contaminates freshwaters and has strong negative effects on aquatic biota. However, both other stressors (e.g. increased sediment) and common ecosystem components (e.g. macrophytes and variable pH) can reduce the aqueous concentrations of malathion, reducing its toxic effects. We conducted a fully orthogonal bioassay to examine how pH (at 7 and 7.8) and sorptive processes (across two levels of kaoline clay 0 and 24 g L -1 ) affected aqueous malathion concentrations and toxicity in an aquatic invertebrate genus. Survival and acetylcholinesterase activity as a sub-organism response were examined in the mayfly Coloburiscoides spp. (Ephemeroptera; Coluburiscidae). Measured aqueous malathion concentrations decreased with increased pH and in the presence of kaolin clay. Survival declined with increasing malathion concentrations and exposure period. Results further identify that antagonism of malathion toxicity was associated with both pH (alkaline hydrolysis) and effects associated with sediment independent of pH (driven by sorptive processes). However, model predictions varied associated with target and measured concentrations and concentrations examined. Antagonistic effects were most apparent using subset target malathion concentrations because of the dominant effect of malathion at high concentrations. Acetylcholinesterase activity, identified repression occurred across all treatments and did not identify antagonistic interactions, but these results were similar to survival responses at the time points examined (i.e. 120 h). Examination of chemistry, acetylcholinesterase, and survival, affords greater understanding of stressor effects and their interactions. Measured malathion concentrations may underestimate effects on aquatic biota; not because of synergism among stressors, but because of strong effects despite antagonism., Competing Interests: Declaration of competing interest We report no conflicts of interest. This is original research, funded by the Australian Research Council (LP160100093) and we are freely available to publish., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Pesticides are increasingly recognised as a threat to freshwater biodiversity, but their specific ecological effects remain difficult to distinguish from those of co-occurring stressors and environmental gradients. Using mesocosms we examined the effects of an organophosphate insecticide (malathion) on stream macroinvertebrate communities concurrently exposed to a suite of stressors typical of streams in agricultural catchments. We assessed the specificity of the SPEcies At Risk index designed to determine pesticide effects in mesocosm trials (SPEAR mesocosm ). This index determines the log abundance proportion of taxa that are considered physiologically sensitive to pesticides. Geographic variation in pesticide sensitivity within taxa, coupled with variation between pesticides and the effects of co-occurring stressors may decrease the accuracy of SPEAR mesocosm . To examine this, we used local pesticide sensitivity assessments based on rapid toxicity tests to develop two new SPEAR versions to compare to the original SPEAR mesocosms index using mesocosm results. We further compared these results to multivariate analyses and community indices (e.g. richness, abundance, Simpson's diversity) commonly used to assess stressor effects on biota. To assess the implications of misclassifying species sensitivity on SPEAR indices we used a series of simulations using artificial data. The impacts of malathion were detectable using SPEAR mesocosm , and one of two new SPEAR indices. All three of the SPEAR indices also increased when exposed to other agricultural non-pesticide stressors, and this change increased with greater pesticide concentrations. Our results support that interactions between other non-pesticide stressors with pesticides can affect SPEAR performance. Multivariate analysis and the other indices used here identified a significant effect of malathion especially at high concentrations, with little or no evidence of effects from the other agricultural stressors., Competing Interests: Declaration of competing interest On behalf of all co-authors, I am reporting that we have no conflicts of interest. This is original research, funded by the Australian Research Council (LP160100093), Australia and we are freely available to publish., (Copyright © 2020 Elsevier B.V. All rights reserved.)

There are limited data on the sensitivity to contaminants of marine organisms in polar regions. Consequently, assessments of the risk of contaminants to marine biota in polar environments typically include extrapolations from temperate and/or tropical species. This is problematic because the taxonomic composition of organisms differs between polar and temperate/tropical waters, and both the toxicity of chemicals and the physiology of organisms are very different at the stable low temperatures experienced in polar marine systems. Collecting high-quality sensitivity data for a wide range of marine polar organisms using traditional toxicity assessment approaches is a time-consuming and difficult process, especially in remote and hostile environments. We applied a rapid toxicity testing approach, which allowed a much larger number of species to be tested than would be possible with traditional toxicity test methods, albeit with lower replications and fewer exposure concentrations. With this rapid approach, sensitivity estimates are less precise, but more numerous. This is important when constructing species sensitivity distributions (SSDs), which aim to represent the sensitivity of communities. We determined the approximate sensitivity (4- and 10-d median lethal concentration [LC50] values) of a large and representative sample of Antarctic marine invertebrates to copper (Cu), zinc (Zn), and cadmium (Cd). Up to 88 LC50 values (from 88 different taxa) were used in the construction of SSDs. The hazardous concentrations for 1% of taxa (HC1) based on 10-d LC50 values were 37, 346, and 792 μg/L for Cu, Zn, and Cd, respectively. Our results provide a basis for estimating the risk of exposure to metals for a large representative sample of marine polar invertebrates. Environ Toxicol Chem 2019;38:1560-1568. © 2019 SETAC., (© 2019 SETAC.)

Determining whether the structural and functional stress responses of communities are similar across space and time is paramount for forecasting and extrapolating the consequences of anthropogenic pressures on ecosystems and their services. Stream ecosystems are under high anthropogenic pressure; however, studies have only examined the response of stream communities across large scales over multiple generations. We studied the responses of leaf-associated microbial communities in streams within three European biogeographical regions to chemical stress in a microcosm experiment with multiple cycles of fungicide pollution and resource colonisation. Fungal community composition and the ecosystem function leaf decomposition were measured as response variables. Microbial leaf decomposition showed similar recovery times under environmental levels of fungicide exposure across regions. Initially, the decomposition declined (between 19 and 53%) under fungicide stress and recovered to control levels during the third cycle of pollution and colonisation. Although community composition and its stress response varied between regions, this suggests similar functional community adaptation towards fungicide stress over time. Genetic, epigenetic and physiological adaptations, as well as species turnover, may have contributed to community adaptation but further studies are required to determine if and to which extent these mechanisms are operating. Overall, our findings provide the first evidence of a similar functional response of microbial leaf decomposition to chemical stress across space and time.

Diffuse agricultural runoff into rivers can result in contamination with herbicides for prolonged periods of time. Chronic exposure to herbicides has the potential to alter toxic impacts in primary producers such as benthic diatoms. Determining how individual diatom taxa respond to herbicide exposure over varied exposure durations is essential for assessing herbicide impacts. This study investigated the responses of various benthic diatom taxa and effects at the community level over 12days of atrazine exposure. Diatom communities were collected from two sites with differing exposure histories; a relatively unpolluted site (Alligator Creek) and an agricultural stream (Barratta Creek) known to be polluted by atrazine and other herbicides. Diatom community composition and the proportion of healthy cells per taxon were assessed at 0, 2, 3, 6, 9 and 12days of atrazine exposure. Pollution history altered the response of the diatom community to atrazine exposure. In the Alligator Creek diatom community there was a shift in composition towards more tolerant taxa and the loss of sensitive taxa in atrazine exposed treatments. The sensitive taxon (Gomphonema truncatum) was consistently affected by atrazine toxicity. Conversely, the polluted Barratta Creek diatom community was not strongly affected by atrazine exposure. Our study shows that during chronic atrazine exposure some taxa demonstrated the ability to recover despite initial toxicity response. Recovery could be an important trait for understanding the ecological effect of herbicide exposure on diatom species in nature and in applied circumstances such as biomonitoring indices., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Ecosystem effects of heavy metals need to be identified for a retrospective risk assessment, and potential impacts need to be predicted for a prospective risk assessment. In this study, we established a strong correlation between the toxic pressure of dissolved metals and invertebrate species. We compiled available data from a wide geographical range of Australian streams that were contaminated with heavy metals [mainly copper (Cu) and zinc (Zn)] and the corresponding invertebrate communities. Heavy metal toxicity is positively related to the proportion of predators within the invertebrate community, represented by the predator ratio , with an effect threshold range of 2.6 μg/L - 26 μg/L for Cu and 62 μg/L - 617 μg/L for Zn. These effect concentrations are in the ranges of the concentrations identified in model ecosystems and other field investigations and are just above the existing guideline limits. Heavy metals also affects the taxa richness negatively. Other community measures, such as the evenness, number of EPT (Ephemeroptera, Plecoptera, and Trichoptera) taxa, SPEcies At Risk (SPEAR) pesticides or SPEAR salinity were relatively poorly correlated with heavy metal toxicity in the streams. Therefore, we suggest applying the predator ratio within the community as a starting point for an indicator of the dissolved metal toxicity, the SPEAR metals ., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Herbicide pollution events in aquatic ecosystems often coincide with increased turbidity and reduced light intensity. It is therefore important to determine whether reduced light intensity can influence herbicide toxicity, especially to primary producers such as benthic diatoms. Benthic diatoms collected from 4 rivers were exposed to herbicides in 48 h rapid toxicity tests under high light (100 µmol m(-2)  s(-1) ) and low light (20 µmol m(-2)  s(-1) ) intensities. The effects of 2 herbicides (atrazine and glyphosate) were assessed on 26 freshwater benthic diatom taxa. There was no significant interaction of light and herbicide effects at the community level or on the majority (22 of 26) of benthic diatom taxa. This indicates that low light levels will likely have only a minor influence on the response of benthic diatoms to herbicides. Environ Toxicol Chem 2016;35:2252-2260. © 2016 SETAC., (© 2016 SETAC.)

Herbicides are common pollutants of rivers in agricultural regions. These contaminants include various types of chemicals with different modes of toxic action. Herbicides can have toxic effects on freshwater benthic diatoms, the base of the aquatic food web. We examined the effects of (non-mixture) herbicide exposure to the health of diatoms for eight common herbicides with three different modes of action; the photosystem II (PSII) inhibitors: atrazine, simazine, hexazinone, tebuthiuron and diuron; two auxinic herbicides: MCPA and 2,4-D; and the EPSP synthase inhibitor: glyphosate. Benthic diatoms within riverine communities were exposed to each herbicide in rapid toxicity tests at concentrations of 50, 200 and 500μgL(-1). The most sensitive taxa were Gomphonema spp. and Encyonema gracilis. Navicula cryptotenella was the most tolerant to herbicide exposure. There was no significant effect of the different herbicide modes of action at the community level. Herbicide mode of action did not alter which taxa were most sensitive within the community and sensitivity rankings of the dominant diatom taxa were similar for each of the eight herbicides. The consistency of the results between herbicides suggests that freshwater benthic diatoms may be suitable in situ indicators for detecting the toxicity of herbicides with differing modes of action., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Herbicides pose a potential threat to aquatic ecosystems, especially to phototrophic organisms such as benthic diatoms. Benthic diatoms may be a valuable indicator of the toxic impacts of herbicides in aquatic systems. However, this requires information on the herbicide sensitivity of a wide range of freshwater benthic diatom taxa. Unfortunately this information is only available for a limited number of species as current methods of developing new algae toxicity tests on individual taxa are lengthy and costly. To address this issue, we developed a new rapid toxicity test method to test natural benthic communities, from which the relative herbicide sensitivity of many individual taxa can be derived. This involved the collection of natural benthic communities from rocks in situ, which were placed directly into laboratory toxicity tests. Sensitivity data for several diatom genera in a 48 hour exposure toxicity test were produced, without the need for cultures or multiple site visits. After exposure to the highest treatment of atrazine (500 μg L(-1)) there were significant declines of healthy cells in the most sensitive genera: Gomphonema declined by 74%, Amphora by 62%, Cymbella by 54% and Ulnaria by 34% compared to control levels. In contrast, the genera, Eunotia, Achnanthidium and Navicula, had no statistically significant decline in cell health. This method can identify the diatom taxa most at risk of herbicide toxicity within the natural benthic diatom community. The rapid toxicity testing method presented is a simple and effective method to obtain sensitivity data for multiple taxa within a natural benthic diatom community in a relatively short period of time., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Bicarbonate is often a major ionic constituent associated with produced waters from methane gas extraction and coal mining, yet few studies have determined its specific toxicity. Currently, the environmental risk of bicarbonate anion in water discharges is assessed based on the toxicity of sodium chloride or artificial seawater and is regulated via electrical conductivity. Increased NaHCO(3) added to Ceriodaphnia dubia in synthetic or natural water gave similar 48-h 10% effective concentration (EC10) values of 1750 ± 125 mg NaHCO(3)/L (mean ± standard error) and 1670 ± 180 mg NaHCO(3)/L, respectively. Bicarbonate was toxic to C. dubia in both waters with conductivities above 1900 µS/cm. In contrast, when conductivity was elevated with NaCl, toxicity to C. dubia was observed only above 2800 µS/cm. Bicarbonate also impaired C. dubia reproduction with an EC10 of 340 mg NaHCO(3)/L. Major ion composition also influenced Zn bioavailability, a common co-occurring metal contaminant in coal mine waters, with sublethal concentrations of NaHCO(3) and elevated pH increasing Zn toxicity. Higher pH was the dominant parameter determining a 10-fold increase in the 48-h 50% effective concentration (EC50) for Zn toxicity to C. dubia at pH 8.6 of 34 µg Zn/L (95% confidence limit = 32-37 µg Zn/L) compared with the Zn toxicity at approximately circumneutral pH. Exposure of the freshwater shrimp Paratya australiensis (Atyidae) in natural water to increasing bicarbonate gave a mean 10-d 10% lethal concentration (LC10) of 850 ± 115 mg NaHCO(3)/L, associated with a mean conductivity EC10 of 1145 µS/cm, which is considerably lower than toxicity of NaCl and artificial seawater to this species reported elsewhere. Because toxicity was influenced by salt composition, specific ions should be regulated rather than conductivity alone in mine wastewater discharges., (© 2014 SETAC.)

Rivers and streams affected by mining activities often receive short-term sharp salinity increases due to water-soluble stockpiled materials being washed into receiving water bodies. We conducted a mesocosm study to explore the response of structural (diatom and stream invertebrate communities) and functional descriptors (chlorophyll a concentration, fungal biomass and leaf decomposition) to repeated short salinity pulses (3h of duration, with nominal electrical conductivities of 5, 10 and 15 mS cm(-1)), mimicking the exposure pattern occurring at salt-mine affected rivers. The experiment was conducted in 12 artificial flow-through stream systems over 16 days. The effect of the salt pulses on the ecosystem structure and functioning did not fully match most of our initial hypotheses, with the community response being weaker than predicted. The diatom community was, however, dominated by salt-tolerant species throughout the experiment, showing no consistent response to the treatment. The invertebrate response was associated with statistically significant changes in community structure (i.e. abundance of the different taxa) but no statistically significant changes in taxa richness. The salt pulses affected some functional descriptors of the ecosystem: fungal biomass exhibited a unimodal response to treatment magnitude, algal growth (i.e. chl a biomass) was hampered with increasing conductivity and leaf decomposition was significantly reduced in the high treatment., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Reliable characterization of exposure is indispensable for ecological risk assessment of chemicals. To deal with mixtures, several approaches have been developed, but their relevance for predicting ecological effects on communities in the field has not been elucidated. In the present study, we compared nine metrics designed for estimating the total toxicity of mixtures regarding their relationship with an effect metric for stream macroinvertebrates. This was done using monitoring data of biota and organic chemicals, mainly pesticides, from five studies comprising 102 streams in several regions of Europe and South-East Australia. Mixtures of less than 10 pesticides per water sample were most common for concurrent exposure. Exposure metrics based on the 5% fraction of a species sensitivity distribution performed best, closely followed by metrics based on the most sensitive species and Daphnia magna as benchmark. Considering only the compound with the highest toxicity and ignoring mixture toxicity was sufficient to estimate toxicity in predominantly agricultural regions with pesticide exposure. The multisubstance Potentially Affected Fraction (msPAF) that combines concentration and response addition was advantageous in the study where further organic toxicants occurred. We give recommendations on exposure metric selection depending on data availability and the involved compounds.

The biodiversity crisis is one of the greatest challenges facing humanity, but our understanding of the drivers remains limited. Thus, after decades of studies and regulation efforts, it remains unknown whether to what degree and at what concentrations modern agricultural pesticides cause regional-scale species losses. We analyzed the effects of pesticides on the regional taxa richness of stream invertebrates in Europe (Germany and France) and Australia (southern Victoria). Pesticides caused statistically significant effects on both the species and family richness in both regions, with losses in taxa up to 42% of the recorded taxonomic pools. Furthermore, the effects in Europe were detected at concentrations that current legislation considers environmentally protective. Thus, the current ecological risk assessment of pesticides falls short of protecting biodiversity, and new approaches linking ecology and ecotoxicology are needed.

Species sensitivity distributions (SSDs) are cumulative distributions of measures of species sensitivity to a stressor or toxicant, and are used to estimate concentrations that will protect p% of a community (PCp ). There is conflict between the desire to use high-quality sensitivity data in SSDs, and to construct them with a large number of species forming a representative sample. Trade-offs between data quality and quantity were investigated using the effects of increasing salinity on the macroinvertebrate community from the Hunter River catchment, in eastern Australia. Five SSDs were constructed, representing five points along a continuum of data quality versus data quantity and representativeness. This continuum was achieved by the various inclusion/exclusion of censored data, nonmodeled data, and extrapolation from related species. Protective concentrations were estimated using the Burr type III distribution, Kaplan-Meier survival function, and two Bayesian statistical models. The dominant taxonomic group was the prime determinant of protective concentrations, with an increase in PC95 values resulting from a decrease in the proportion of Ephemeropteran species included in the SSD. In addition, decreases in data quantity in a SSD decreased community representativeness. The authors suggest, at least for salinity, that the inclusion of right censored data provides a more representative sample of species that reflects the natural biotic assemblage of an area to be protected, and will therefore improve risk assessment., (Copyright © 2013 SETAC.)

In flowing water pollution regularly occurs in short pulses (hours to days). Populations of species affected by pulses have the potential to recover in the absence of further disturbance but recovery rates will vary between species due to resilience (e.g. generation time and dispersal ability) and avoidance traits. Current assessments of the risks of chemicals to community structure--predominantly based on species sensitivity distributions (SSDs)--only consider physiological sensitivity and do not give any consideration as to the rate at which populations will recover. We constructed SSDs of ecologically sensitive and tolerant stream invertebrate assemblages (based on 3 traits previously shown to be important in determining how species relative abundances respond to pesticide toxicity) from south-east Australia and in regions of Finland, Germany and France. There were differences in SSDs of a generic measure of physiological sensitivity to organic chemicals between ecologically sensitive and tolerant species, though these differences were not consistent between the regions studied. We conclude that it is important for community level risk assessments of pulses of chemicals that the ecological sensitivity of the regional species assemblage is considered and discuss several options as to how this could be achieved., (Copyright © 2012 Elsevier B.V. All rights reserved.)

Salinization of freshwater ecosystems is a global problem affecting many regions worldwide and can co-occur with pesticides in agricultural regions. Given that both stressors are potent to affect macroinvertebrate communities, their effects could interact. We investigated the effects of salinity and pesticides at 24 sites in an agricultural region of southern Victoria, South-East Australia. We used distance-based redundancy analysis to determine the influence of pesticides, salinity and other environmental variables on the composition of macroinvertebrate communities. Salinity and pesticide toxicity had a statistically significant effect on communities as had the substrate composition and the percentage of pool and riffle sections in the sampled stream reaches. We did not find evidence for interactive effects between salinity and pesticides, i.e. the effect of one of these variables did not depend on the level of the other. Nevertheless, our results show that salinization and exposure to pesticides can be major factors for the structure of macroinvertebrate communities in agricultural regions. Pesticide toxicity acted on a lower taxonomic level compared to salinity, potentially indicating evolutionary adaptation to salinity stress., (Copyright © 2012 Elsevier B.V. All rights reserved.)

We compiled data from eight field studies conducted between 1998 and 2010 in Europe, Siberia, and Australia to derive thresholds for the effects of pesticides on macroinvertebrate communities and the ecosystem function leaf breakdown. Dose-response models for the relationship of pesticide toxicity with the abundance of sensitive macroinvertebrate taxa showed significant differences to reference sites at 1/1000 to 1/10,000 of the median acute effect concentration (EC50) for Daphnia magna, depending on the model specification and whether forested upstream sections were present. Hence, the analysis revealed effects well below the threshold of 1/100 of the EC50 for D. magna incorporated in the European Union Uniform Principles (UP) for registration of single pesticides. Moreover, the abundances of sensitive macroinvertebrates in the communities were reduced by 27% to 61% at concentrations related to 1/100 of the EC50 for D. magna. The invertebrate leaf breakdown rate was positively linearly related to the abundance of pesticide-sensitive macroinvertebrate species in the communities, though only for two of the three countries examined. We argue that the low effect thresholds observed were not mainly because of an underestimation of field exposure or confounding factors. From the results gathered we derive that the UP threshold for single pesticides based on D. magna is not protective for field communities subject to multiple stressors, pesticide mixtures, and repeated exposures and that risk mitigation measures, such as forested landscape patches, can alleviate effects of pesticides., (© 2012 American Chemical Society)

Effects of anthropogenic and environmental stressors on freshwater communities can propagate to ecosystem functions and may in turn impede ecosystem services. We investigated potential shifts in ecosystem functions that provide energy for freshwater ecosystems due to pesticides and salinity in 24 sites in streams of southeast Australia. First, effects on allochthonous organic matter (AOM) breakdown using three different substrates (leaves, cotton strips, wood sticks) in coarse and fine bags were investigated. Second, we examined effects on stream metabolism that delivers information on the ecosystem functions of gross primary production and ecosystem respiration. We found up to a fourfold reduction in AOM breakdown due to exposure to pesticides and salinity, where both stressors contributed approximately equally to the reduction. The effect was additive as, no interaction or correlation between the two stressors was found. Leaf breakdown responded strongly and exclusively to exposure to pesticides and salinity, whereas cotton strip breakdown was less sensitive and responded also to other stressors such as nutrients. No functional redundancy for the effects of pesticides and salinity on leaf breakdown was observed. For wood stick breakdown, no relationship to environmental gradients was found, however, the sample size was lower. We did not detect effects of pesticides or salinity on gross primary production or ecosystem respiration. A reduction in AOM breakdown by pesticides and salinity may impair the ecosystem services of food provision and possibly water purification. Hence, future studies should examine the spatial extent of these effects., (Copyright © 2011 Elsevier B.V. All rights reserved.)

Agricultural pesticides are widely used and can affect freshwater organisms. We applied a spatially explicit exposure model, validated for central Europe, to estimate exposure to insecticides through runoff for streams in south-eastern Australia. The model allows the identification of streams potentially affected by insecticide runoff located in 10×10 km grid cells. The computation of runoff relies on key environmental factors such as land use, soil texture, slope and precipitation. Additionally, the model predicted the ecological effect of insecticides on the macroinvertebrate community. We predicted insecticide surface runoff that results in a moderate to poor ecological quality for streams in half of the grid cells containing agricultural land. These results are in good accordance with the results obtained by estimating pesticide stress with a biotic index (SPEAR(pesticides)) based on macroinvertebrate monitoring data. We conclude that the exposure and effect model can act as an effective and cost-saving tool to identify high risk areas of insecticide exposure and to support stream management., (Copyright © 2011 Elsevier B.V. All rights reserved.)

We compiled a database on a priori selected traits for South-East Australian freshwater macroinvertebrate families and used this data for the development of a biotic indicator for the detection of the effects of salinisation on freshwater communities (SPEAR(salinity)) and for the adaptation of the existing SPEAR(pesticides) index for South-East Australian taxa. The SPEAR(salinity) indicator showed a reasonably high relationship (0.38≤r(2)≤0.5) with salinity in terms of logarithmic electrical conductivity (log EC) using field biomonitoring data from 835 pools and riffle sites in Victoria and South Australia. Several other biotic indexes that were calculated for comparison purpose exhibited a lower relationship with log EC. In addition, SPEAR(salinity) was the only indicator that did not respond to other water quality variables and was therefore most selective. We used log EC data and modelled pesticide exposure for sites in Victoria in concert with SPEAR(salinity) and the existing SPEAR(pesticides) index to assess whether pesticides interacts with effects of salinity on invertebrate communities and vice versa. No interaction with pesticides was found for the effect of log EC on SPEAR(salinity), whereas EC interacted with the estimated pesticide exposure on the invertebrate communities. To foster the development of further trait-based ecological indicators, we suggest a conceptual model that predicts response traits based on the disturbance regime and disturbance mode of action of the stressor. Biotic indicators based on a priori selected traits represent a promising biomonitoring tool even for regions where ecological information is scarce., (Copyright © 2011 Elsevier B.V. All rights reserved.)

Grab water samples, sediment samples, and 2,2,4-trimethylpentane passive samplers (TRIMPS) were used to determine the exposure to 97 pesticides in 24 southeast Australian stream sites over 5 months. Macroinvertebrate communities and selected microorganisms (bacteria, flagellates, ciliates, amoebas, nematodes, and gastrotrichs) were sampled to detect relationships with pesticide toxicity. Sediment samples had the highest estimated toxicities in terms of toxic units (TU) for Daphnia magna (TUDM) and for Selenastrum capricornutum (TUSC). The pesticide-selective SPEARpesticides and the general SIGNAL index for macroinvertebrates exhibited negative linear relationships (r(2) = 0.67 and 0.36, respectively) with pesticide contamination in terms of log maximum TUDM (log mTUDM), suggesting macroinvertebrate community change due to pesticide exposure. Pesticide contamination was the only measured variable explaining variation in ecological quality. Variation in the densities of several microbial groups was best explained by environmental variables other than log TUs. The log mTUDM values derived from sediment concentrations were most important to establish a link with effects on macroinvertebrates, whereas log mTUDM of grab water samples had only minor contribution. Current-use insecticides and fungicides can affect macroinvertebrate communities and monitoring of sediment and continuous water sampling is needed to detect these effects.

Salinisation of freshwater has been identified as a serious environmental issue in Australia and around the world. Protective concentrations (trigger values) for salinity can be used to manage salinity impacts, though require locally relevant salinity tolerance information. 72-h acute salinity tolerance values were determined for 102 macroinvertebrates collected from 11 locations in four biologically distinct freshwater bio-regions in Northeast Australia and compared with sensitivities observed in Southeast Australia. The salinity tolerance of individual taxa was consistent across Northeast Australia and between Northeast and Southeast Australia. However, two distinct communities were identified in Northeast Australia using distributions of the acute tolerance values and a calculated index of salinity sensitivity. Salinity trigger values should therefore be representative of local or regionally relevant communities and may be adequately calculated using sensitivity values from throughout Eastern Australia. The results presented provide a basis for assessing salinity risk and determining trigger values for salinity in freshwater ecosystems at local and regional scales in Eastern Australia.

Increasing salinity has the potential to affect freshwater organisms. Yet sub-lethal effects of salinity on macroinvertebrates are poorly understood. Growth and development of Ischnura heterosticta (Odonata: Coenagrionidae) was experimentally shown to be faster in 5-20 mS/cm than 0.1-1 mS/cm, while in 35 mS/cm all individuals died. In 30 mS/cm about half died and growth was similar to the 0.1 mS/cm treatment. The salinity-growth relationship cannot be explained indirectly, that is salinity affecting the survival of their prey. Tissue content and concentration of Ca, Mg, Na and K in emerged adults showed no evidence of deficiencies at low salinity. Heart beat rate was similar across treatments, except at 35 mS/cm, where it was slower. Respiration and feeding were similar at 0.1, 10 and 30 mS/cm. While there are similarities in I. heterosticta and other species' salinity response, there are differences and studies on more species are urgently needed.