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Abstract The Central Andes of South America boasts a rich history of complex societies and sophisticated economic networks. Reconstructing patterns of diet across time is important to understanding the relationships between subsistence and food preparation and their roles in mediating consumption and hegemony through time. The region surrounding the city of Cusco in the southern Peruvian highlands is best known as the heartland of the Inca Empire (520-418 BP); however, it has a long history of social complexity and regional exchange, including colonization by the highland Wari Empire (1350-950 BP) and in situ development in earlier periods. Elucidating subsistence and mobility over time in the Cusco region is therefore essential for reconstructing the evolution of complex Andean polities and their effects on local communities. This study presents carbon, nitrogen, and oxygen isotopic data from human bone and tooth enamel at four Cusco-region sites: the hunter-gatherer site of Kasapata (6350- 4150 BP, N = 8); the village site of Yuthu (2350-2050 BP, N = 22); the Wari colony of Hatun Cotuyoc (1350-950 BP, N = 9) and the contemporaneous village site of Ak'awillay (N = 22). Key aims are to estimate diachronic shifts in foodways and nutrition, and those related to Wari control. Results indicate nearly-identical isotope values at Kasapata and Yuthu trending toward lower-trophic level C 3 proteins and C 3 energy sources, while values indicate mixed C 3 /C 4 diets at Ak'awillay and diets dominated by terrestrial meat and C 4 foods at Hatun Cotuyoc. Interestingly, oxygen isotope values suggest water source variation consistent with minimal mobility at Kasapata and regional mobility at Yuthu, but possibly with overlapping but divergent foodways at Ak'awillay and Hatun Cotuyoc resulting in differential evaporative pressures on consumed water rather than increased mobility. Highlights • This study represents the first isotopic study of pre-Inca sites in the Cusco region. • This study presents a rare isotopic paleodiet reconstruction of Archaic Period Andean hunter-gatherers. • Carbon, nitrogen, and oxygen isotopes characterized to estimate diet and source water consumption. • Results suggest diet shifts between Archaic & Formative and Middle Horizon periods. • Results highlight potential differences in foodways in the Middle Horizon, mediated by Wari control. [ABSTRACT FROM AUTHOR]

Highlights: [•] Distributions of state canons demonstrate power of ancient states archaeologically. [•] Regional survey data in Cusco, Peru, reveal large-scale Wari interaction patterns. [•] Survey data show that Wari power influenced only certain parts of the Cusco region. [•] GIS analysis of ceramic distribution indicates uneven and indirect Wari influence. [•] Wari regional power in Cusco suggests colonization rather than provincial rule. [Copyright &y& Elsevier]