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Background: Genetic variants in voltage-gated sodium channels (Na v ) encoded by SCNXA genes, responsible for I Na , and K v 4.3 channels encoded by KCND3 , responsible for the transient outward current (I to ), contribute to the manifestation of both Brugada syndrome (BrS) and spinocerebellar ataxia (SCA19/22). We examined the hypothesis that K v 4.3 and Na v variants regulate each other's function, thus modulating I Na /I to balance in cardiomyocytes and I Na /I (A) balance in neurons., Methods: Bicistronic and other constructs were used to express WT or variant Na v 1.5 and K v 4.3 channels in HEK293 cells. I Na and I to were recorded., Results: SCN5A variants associated with BrS reduced I Na , but increased I to . Moreover, BrS and SCA19/22 KCND3 variants associated with a gain of function of I to , significantly reduced I Na , whereas the SCA19/22 KCND3 variants associated with a loss of function (LOF) of I to significantly increased I Na . Auxiliary subunits Na v β1, MiRP3 and KChIP2 also modulated I Na /I to balance. Co-immunoprecipitation and Duolink studies suggested that the two channels interact within the intracellular compartments and biotinylation showed that LOF SCN5A variants can increase K v 4.3 cell-surface expression., Conclusion: Na v and K v 4.3 channels modulate each other's function via trafficking and gating mechanisms, which have important implications for improved understanding of these allelic cardiac and neuronal syndromes.