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Seventeen subjects with more than 3 x 10(5) mutans streptococci per millilitre of saliva completed this randomised, cross-over study. Four different chewing-gums, containing: (1) 70% xylitol, (2) 35% xylitol + 35% sorbitol, (3) 17.5% xylitol + 52.5% sorbitol, and (4) 70% sorbitol, were tested. The participants used 12 pieces of each gum per day for 25 days. During the four experimental periods, they wore a removable palatinal plate containing two demineralised enamel samples, and brushed their teeth with a non-fluoridated toothpaste. The results showed that an increased concentration of xylitol in the gum resulted in a lower number of mutans streptococci in both saliva and dental plaque, although the decreases were only significant in the saliva samples (p

In this paper, data are presented on the in situ demineralization of human enamel as a function of the demineralization period. To quantify the mineral loss parameters versus time, it is important to obtain information on the kinetics, and thus on the mechanism of dental caries. The results show that for in situ enamel demineralization, the lesion depth as well as the mineral loss parameter both vary linearly with the demineralization time. This is in contrast to in vitro lesion formation where the third power, or the square power of the lesion depth is linearly related to the demineralization time. In in situ demineralization, the rate-determining step of the demineralization process is the inhibitor-controlled dissolution process at the enamel crystallite surfaces, while the inhibitor content (F-, proteins etc.) in the lesion originating from the plaque, saliva and enamel is high. Furthermore, the study indicates that in in situ demineralization, interprismatic mineral loss is very important.

The in situ remineralization of 100-microns-deep enamel lesions positioned in a full prosthesis just under the first molar was determined in 13 participants after 3 months. The outer surface of the samples was always flush with the acrylic surface: there was no measurable plaque accumulation on the samples. The effect of not brushing of the samples (A) is compared with the effect of brushing with a nonfluoridated dentifrice (B) or with a fluoridated paste (C) containing 1,250 ppm F. The results show that lesion depth (and mineral loss) data increase by about 50% (case A), do not change statistically in case B and decrease by about 40% in case C. The increase of lesion depth in case A is most likely due to acidic food and/or beverage intake by the participants. With respect to nonbrushing, the remineralization effect of brushing with an F-free paste is about 50% and with the fluoridated paste about 90% in 3 months. It is suggested that the remineralization efficacy of fluoridated pastes is due to the cleaning effect of the brushing by the dentifrice (presumably on the pellicle) as well as due to the fluoride effect on mineral nucleation and growth.

In this in vivo study the amount of fluoride present on and in human enamel was followed longitudinally for a period of 3 months. The fluoridating agents were an APF gel and the fluoridating lacquers

A single topical treatment with three different fluoride-containing systems on human enamel resulted both in an enrichment of the F content in the enamel and an accumulation of F re

Shallow initial enamel lesions approximately 50 μm in depth were experimentally induced by plaque in sound intact human enamel. The lesions were subsequently remineralized in vivo without any fluoride

The aim of this study was to compare the rate of etching and the surface roughness of HClO4 etched enamel surfaces. The average thickness of the enamel layer removed is a linear function of the total etching period (except for very short periods). The rate of etching (β) in μm s––1 with HCIO4 is strongly influenced by acid strength and stirring rate during etching; it is ∼ 0.02, 0.06, 0.18 and 0.74 μm s––1 for 0.02, 0.1, 0.5 and 2.0 M HCIO4, respectively, β is a linear function of the acid strength. Scanning electron microscopy (SEM) pictures of the specimens show that acid strength, etching time and chemical composition of the enamel are most likely important factors determining surface morphology of etched enamel. A lower acid concentration gives a sharper etch; increasing acid strength causes increasing surface roughness.

Three patients with a partial natural dentition in one jaw and a full denture in the other participated in this study. Enamel treated with APF gel, Duraphat or Fluor Protector and untreated controls were positioned in a full denture for periods up to 3 months. Plaque accumulation on the enamel blocks was evaluated by scanning electron microscopy. The results show that in all 3 patients studied the formation of CaF2 inhibited dental plaque accumulation.