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A patent application titled "Methods And Constructs For Production Of Lentiviral Vector" was published online by inventors from Pearland, TX. The application describes methods for producing lentiviral packaging and vector-producing mammalian cells, aiming to simplify and enhance lentiviral vector production. The application outlines the use of specific promoters and transposon-specific inverted terminal repeats to integrate genes required for lentiviral vector production into mammalian cells. This innovation could potentially improve the efficiency and safety of lentiviral vector production for gene and cell therapy applications. [Extracted from the article]

A recent study conducted by researchers at the University of California has provided new insights into the structural heterogeneity of the rabies virus. The study utilized cryogenic electron tomography and microscopy to determine the three-dimensional structure of the wild-type rabies virus. The researchers found that the virus exhibits a high level of structural heterogeneity, particularly in the glycoprotein trimer-decorated envelope and the nucleocapsid composed of RNA, nucleoprotein, and matrix protein. These findings suggest different mechanisms of viral assembly compared to other rhabdoviruses. This research contributes to our understanding of the rabies virus and may have implications for the development of viral vaccines. [Extracted from the article]

The article focuses on the use of the Product Enhanced Reverse Transcriptase (PERT) assay for detecting replication-competent viruses in retroviral vectors. Topics include the comparison of PERT with the S+/L- assay, the effectiveness of PERT in distinguishing virus-free from virus-containing vector products, and the application of PERT for vectors pseudotyped with GALV and VSVG envelopes.