Infectious diseases normally tend to have very strong infectivity, and be easy to widely spread in a large geographical area and difficult to control. Therefore, they cause serious harm to people’s life and health. Rapid and accurate screening has become the urgent need for the prevention and control of their prevalence. Micro RNAs(mi RNAs) are a class of small noncoding RNAs(about 22 nucleotides, nt), commonly found in eukaryotic cells. Mi RNAs play critical roles in a variety of physiological and pathological processes including cell growth, development and apoptosis, tumorigenesis and virus infection by regulating gene expression or post-transcriptional translation, which work by perfect or imperfect base pairing between short seed regions of mi RNAs and 3’ UTR of target m RNAs. During virus infection, mi RNAs are documented to have crucial impact on the interaction between viruses and hosts. Interestingly, the specific expressions of mi RNAs make them expected to become clinical markers for screening virus infectious diseases, which are emerging as a hot research field. This paper summarizes the stability of mi RNAs in human body fluids, reviews the specificity of mi RNAs expression in virus infection and the methods of mi RNAs detection, and performs the feasibility analysis about mi RNA as a group of novel and potential biomarkers of virus-related infectious diseases. [ABSTRACT FROM AUTHOR]