1. Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis.
- Author
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Thwaites RS, Ito K, Chingono JMS, Coates M, Jarvis HC, Tunstall T, Anderson-Dring L, Cass L, Rapeport G, Openshaw PJ, Nadel S, and Hansel TT
- Subjects
- Case-Control Studies, Chemokine CCL5 analysis, Female, Humans, Infant, Interferon-gamma analysis, Interleukins analysis, London, Male, Nasal Mucosa virology, Respiratory Syncytial Virus Infections virology, Respiratory Syncytial Virus, Human, Bronchiolitis, Viral diagnosis, Inflammation virology, Nasal Mucosa immunology, Respiratory Syncytial Virus Infections diagnosis, Viral Load methods
- Abstract
Background: Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting., Methods: Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)-positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA)., Results: Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05)., Conclusions: Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants., (© The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America.)
- Published
- 2017
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