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Copyright of Journal of Shaanxi University of Science & Technology is the property of Journal of Shaanxi University of Science & Technology Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shaanxi University of Science & Technology is the property of Journal of Shaanxi University of Science & Technology Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Vibrio alginolyticus is one of the important fish pathogen in China,bringing about serious economic loss to marine culture. In this paper,a sRNA,RyhB,was screened with the bioinformatics methods. The gene encoded for RyhB was cloned and the in-frame deletion mutant strain was constructed in Vibrio alginolyticus. Also the functions of RyhB in in Vibrio alginolyticus were characterized. The results showed that the gene encoded for RyhB is 233bp in size,demonstrated the classical stem-loop structure and contained the Fur binding sites in -10 to -35 region. There are multiple potential target mRNAs of RyhB. indicating that RyhB may be involved in many physiological processes in Vibrio alginolyticus. Besides. the ability of mobility and the siderophore production were both impaired in the A ryhB mutant strain,contrast to the wild-type strain. This paper will enrich the people's recognition of the sRNA and virulence regulation mechanism in Vibrio alginolyticus. [ABSTRACT FROM AUTHOR]