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Quorum sensing (QS), acts as one of the gene regulatory systems that allow bacteria to regulate their physiological activities by sensing the population density with synchronization of the signaling molecules that they produce. Here, we report a marine isolate, namely strain T47, and its unique AHL profile. Strain T47 was identified using 16S rRNA sequence analysis confirming that it is a member of Vibrio closely clustered to Vibrio sinaloensis. The isolated V. sinaloensis strain T47 was confirmed to produce N-butanoyl-L-homoserine lactone (C4-HSL) by using high resolution liquid chromatography tandem mass spectrometry. V. sinaloensis strain T47 also formed biofilms and its biofilm formation could be affected by anti-QS compound (cathechin) suggesting this is a QS-regulated trait in V. sinaloensis strain T47. To our knowledge, this is the first documentation of AHL and biofilm production in V. sinaloensis strain T47. more...

N-acylhomoserine lactones (AHL) plays roles as signal molecules in quorum sensing (QS) in most Gram-negative bacteria. QS regulates various physiological activities in relation with population density and concentration of signal molecules. With the aim of isolating marine water-borne bacteria that possess QS properties, we report here the preliminary screening of marine bacteria for AHL production using Chromobacterium violaceum CV026 as the AHL biosensor. Strain T33 was isolated based on preliminary AHL screening and further identified by using 16S rDNA sequence analysis as a member of the genus Vibrio closely related to Vibrio brasiliensis. The isolated Vibrio sp. strain T33 was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL) and N-(3-oxodecanoyl)-L-homoserine lactone (3-oxo-C10 HSL) through high resolution tandem mass spectrometry analysis. We demonstrated that this isolate formed biofilms which could be inhibited by catechin. To the best of our knowledge, this is the first report that documents the production of these AHLs by Vibrio brasiliensis strain T33. more...

Vibriospp. have been widely studied for their unique properties such as pathogenicity and quorum sensing (QS) abilities. This article presents the identification ofVibrio variabilisstrain T01 isolated from Malaysian coastal waters. Strain T01 ofV. variabiliswas identified to produce QS molecules as tested using a biosensor. High-resolution tandem mass spectrometry was used to identifyN-acyl homoserine lactone (AHL) profiles of strain T01. Three AHLs,viz. N-hexanoyl-L-homoserine lactone (C6-HSL),N-(3-oxodecanoyl)-L-homoserine lactone (3-oxo-C10-HSL) andN-dodecanoyl-L-homoserine lactone (C12-HSL), were confirmed. To the authors' knowledge, this is the first documentation of AHL profiles fromV. variabilisstrain T01, which expands the number of QS members inVibriospp. [ABSTRACT FROM AUTHOR] more...