20 results on '"Wang, Yonglin"'
Search Results
2. Development of Integrated Control for Verticillium Wilt of Smoke Trees in Beijing.
- Author
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Li, Bimeng, Guo, Ruifeng, Zhao, Yize, Li, Qiyan, Song, Lizhou, Shen, Chong, Du, Chenming, Gu, Yuntao, Qiao, Guanghang, Wang, Liping, Yuan, Fei, Huang, Sanxiang, and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,VERTICILLIUM wilt diseases ,ORNAMENTAL trees ,SMOKE ,FOREST reserves ,CARBENDAZIM ,PROPICONAZOLE - Abstract
Smoke tree (Cotinus coggygria) is an important ornamental tree that represents the autumnal landscape of red leaves in Northern China, especially in Beijing. However, Verticillium wilt, caused by the fungus (Verticillium dahliae), has resulted in a high mortality rate for smoke trees, posing a serious threat to the highly valued landscape of red leaves in Beijing. To explore an efficient control measure for Verticillium wilt, we systematically analyzed the applicability and efficacy of multiple treatments for three consecutive years in Xiangshan Park and Badaling Forest Park. From 2021 to 2023, diseased smoke trees in Xiangshan Park were subjected to three application methods (agent irrigation, trunk injection, or a combination of the two) and five candidate agents, namely Bacillus subtilis, azoxystrobin, propiconazole, carbendazim, and prochloraz. Analyses of the data for three consecutive years revealed a decreasing trend in the annual disease incidence rate. Specifically, the combined application of agent irrigation and trunk injection exhibited the highest control effect and a significant improvement in the landscape of red leaves in Beijing. Furthermore, the combination of propiconazole via irrigation plus the trunk injection of carbendazim and prochloraz had the greatest control effect. These suppressive measurements were further used and demonstrated to be effective in Badaling Forest Park. Overall, our study provides an effective disease management means for controlling Verticillium wilt in smoke trees. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. CRISPR‐based platforms for the specific and dual detection of defoliating/nondefoliating strains of Verticillium dahliae.
- Author
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Chen, Qi, Wu, Jin, Tang, Chen, and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,VERTICILLIUM wilt diseases ,CRISPRS ,RAPID tooling ,PATHOGENIC fungi ,PLANT species - Abstract
BACKGROUND: Verticillium dahliae is a soil‐borne pathogenic fungus that causes Verticillium wilt disease on more than 400 plant species worldwide. Because of its broad host range and its ability to survive long term in the soil, there are few effective control measures for V. dahliae once it has become established. Accurate, sensitive, and rapid detection of V. dahliae is crucial for limiting pathogen entry into new regional environments and early management of Verticillium wilt. RESULTS: In this study, we developed a method to detect V. dahliae based on recombinase polymerase amplification (RPA) and CRISPR/Cas technology and used fluorescence and lateral flow test strips to monitor the outcomes. Through the establishment and optimization of RPA–CRISPR/Cas13a detection, the sensitivity of the fluorescence method was 1 am for genomic DNA (gDNA) within 20 min, whereas the sensitivity of the lateral flow strip method was 100 am for gDNA in 30 min. The field applicability of RPA–CRISPR/Cas13a was also validated by the detection of V. dahliae on smoke trees (Cotinus coggygria) in Xiangshan Park, Beijing, China. Finally, diplex detection for defoliating and nondefoliating pathotypes of V. dahliae was established by combining CRISPR–Cas12a/Cas13a with specific target genes. CONCLUSION: Taken together, this study achieved rapid, sensitive, and accurate detection of V. dahliae and the differentiation of defoliating and nondefoliating pathotypes and provides potential for field‐deployable diagnostic tools for rapid and ultrasensitive detection. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Deletion of VdKu80 enhances targeted gene replacement in Verticillium dahliae
- Author
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Xiong, Dianguang, Deng, Chenglin, Wang, Yonglin, and Tian, Chengming
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- 2018
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5. Transcriptomic profiles of the smoke tree wilt fungus Verticillium dahliae under nutrient starvation stresses
- Author
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Xiong, Dianguang, Wang, Yonglin, and Tian, Chengming
- Published
- 2015
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- View/download PDF
6. Genetic transformation, infection process and qPCR quantification of Verticillium dahliae on smoke-tree Cotinus coggygria
- Author
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Wang, Yonglin, Xiao, Shuxiao, Xiong, Dianguang, and Tian, Chengming
- Published
- 2013
- Full Text
- View/download PDF
7. Transcriptome Variations in Verticillium dahliae in Response to Two Different Inorganic Nitrogen Sources.
- Author
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Tang, Chen, Li, Wenwen, Klosterman, Steven J., and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,ORGANONITROGEN compounds ,NITRATE reductase ,WILT diseases ,AMMONIUM nitrate ,RNA polymerases - Abstract
The fungus Verticillium dahliae causes vascular wilt disease on hundreds of plant species. The main focus of the research to control this fungus has been aimed at infection processes such as penetration peg formation and effector secretion, but the ability of the fungus to acquire and utilize nutrients are often overlooked and may hold additional potential to formulate new disease control approaches. Little is known about the molecular mechanisms of nitrogen acquisition and assimilation processes in V. dahliae. In this present study, RNA sequencing and gene expression analysis were used to examine differentially expressed genes in response to the different nitrogen sources, nitrate and ammonium, in V. dahliae. A total of 3244 and 2528 differentially expressed genes were identified in response to nitrate and ammonium treatments, respectively. The data indicated nitrate metabolism requires additional energy input while ammonium metabolism is accompanied by reductions in particular cellular processes. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of DEGs during nitrate metabolism revealed that many of the genes encoded those involved in protein biosynthetic and metabolic processes, especially ribosome and RNA polymerase biosynthesis, but also other processes including transport and organonitrogen compound metabolism. Analysis of DEGs in the ammonium treatment indicated that cell cycle, oxidoreductase, and certain metabolic activities were reduced. In addition, DEGs participating in the utilization of both nitrate and ammonium were related to L-serine biosynthesis, energy-dependent multidrug efflux pump activity, and glycerol transport. We further showed that the mutants of three differentially expressed transcription factors (VdMcm1 , VdHapX , and VDAG_08640) exhibited abnormal phenotypes under nitrate and ammonium treatment compared with the wild type strain. Deletion of VdMcm1 displayed slower growth when utilizing both nitrogen sources, while deletion of VdHapX and VDAG_08640 only affected nitrate metabolism, inferring that nitrogen assimilation required regulation of bZIP transcription factor family and participation of cell cycle. Taken together, our findings illustrate the convergent and distinctive regulatory mechanisms between preferred (ammonium) and alternative nitrogen (nitrate) metabolism at the transcriptome level, leading to better understanding of inorganic nitrogen metabolism in V. dahliae. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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- View/download PDF
8. Convergent and distinctive functions of transcription factors VdYap1, VdAtf1, and VdSkn7 in the regulation of nitrosative stress resistance, microsclerotia formation, and virulence in Verticillium dahliae.
- Author
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Tang, Chen, Jin, Xianjiang, Klosterman, Steven J., and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,TRANSCRIPTION factors ,PHYTOPATHOGENIC microorganisms ,PATHOGENIC fungi ,VERTICILLIUM wilt diseases ,NURSES - Abstract
Reactive oxygen/nitrogen species (ROS/RNS) play a fundamental role in plant–fungal interactions. How pathogenic fungi manipulate plant‐derived ROS/RNS is of importance to the outcomes of these interactions. In this study, we explored the individual and combined contributions of three transcription factors, VdAtf1, VdYap1, and VdSkn7, in the response to ROS/RNS, microsclerotia formation, and virulence in the plant wilt pathogen Verticillium dahliae. We showed that VdYap1 is essential for ROS response. Additionally, mutants lacking any combination of the three genes shared significant hypersensitivity to nitro‐oxidative stress like sodium nitroprusside dehydrate and double deletions lacking VdYap1 and VdAtf1 resulted in further increased sensitivity to ROS. Double deletion of VdAtf1 and VdSkn7 reduced melanin production and virulence while simultaneous lack of VdSkn7 and VdYap1 disrupted nitrogen metabolism and ROS resistance. Finally, comparison of transcriptional profiles of the respective single or double mutants in response to nitro‐oxidative stress revealed that the three transcription factors are involved in denitrification of nitrated alkanes and lipids to protect against nitro‐oxidative stress. Taken together, our results demonstrate convergent and distinctive functions of VdYap1, VdAtf1, and VdSkn7 in V. dahliae, and provide new data on their roles in response to ROS/RNS in fungi. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
9. The bZIP transcription factor VdAtf1 regulates virulence by mediating nitrogen metabolism in Verticillium dahliae.
- Author
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Tang, Chen, Li, Tianyu, Klosterman, Steven J., Tian, Chengming, and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,TRANSCRIPTION factors ,FUNGAL cell walls ,DELETION mutation ,NITRIC oxide regulation ,WILT diseases - Abstract
Summary: The fungus Verticillium dahliae causes vascular wilt disease on hundreds of plant species. Homologs of the bZIP transcription factor Atf1 are required for virulence in most pathogenic fungi, but the molecular basis for their involvement is largely unknown.We performed targeted gene deletion, expression analysis, biochemistry and pathogenicity assays to demonstrate that VdAtf1 governs pathogenesis via the regulation of nitrosative resistance and nitrogen metabolism in V. dahliae.VdAtf1 controls pathogenesis via the regulation of nitric oxide (NO) resistance and inorganic nitrogen metabolism rather than oxidative resistance and is important for penetration peg formation in V. dahliae. VdAtf1 affects ammonium and nitrate assimilation in response to various nitrogen sources. VdAtf1 may be involved in regulating the expression of VdNut1. VdAtf1 responds to NO stress by strengthening the fungal cell wall, and by causing over‐accumulation of methylglyoxal and glycerol, which in turn impacts NO detoxification. We also verified that the VdAtf1 ortholog in Fusarium graminearum mediates nitrogen metabolism, suggesting conservation of this function in related plant pathogenic fungi.Our findings revealed new functions of VdAtf1 in pathogenesis, response to nitrosative stress and nitrogen metabolism in V. dahliae. The results provide novel insights into the regulatory mechanisms of the transcription factor VdAtf1 in virulence. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
10. Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae.
- Author
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Fang, Yulin, Klosterman, Steven J., Tian, Chengming, and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,HIGH temperatures ,DENATURATION of proteins ,WILT diseases ,IRRADIATION - Abstract
Summary: The fungus Verticillium dahliae causes vascular wilt disease on more than 200 plant species worldwide. This fungus can survive for years in soil as melanized microsclerotia. We found that VdCmr1, a transcription factor, is required for the melanin production and increased survival following UV irradiation in V. dahliae but not for microsclerotia production or virulence. Here, we provided evidence how VdCmr1 protects against high temperature (HT) and UV irradiation in V. dahliae. The results indicate that VdCmr1 mediates entry to the diapause period in V. dahliae in response to HT and contributes to the expression of proteins to minimize protein misfolding and denaturation. VdCmr1 deletion results in the misregulation of DNA repair machinery, suggestive of reduced DNA repair capacity following UV irradiation and in correlation with the low survival rate of UV‐treated VdCmr1 mutants. We discovered a putative VdCmr1‐dependent gene cluster associated with secondary metabolism and stress responses. We also functionally characterized two VdCmr1‐responsive genes participating in HT and UV response. These results shed further light on the roles of VdCmr1 in protection from HT or UV irradiation, and the additional insights into the mechanisms of this protection may be useful to exploit for more effective disease control. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
11. Involvement of a Response Regulator VdSsk1 in Stress Response, Melanin Biosynthesis and Full Virulence in Verticillium dahliae.
- Author
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Zheng, Jiayue, Tang, Chen, Deng, Chenglin, and Wang, Yonglin
- Subjects
VERTICILLIUM dahliae ,MELANINS ,BIOSYNTHESIS ,WILT diseases ,FUNGICIDE resistance ,PSYCHOLOGICAL stress - Abstract
Verticillium dahliae causes vascular wilt disease on over 200 plant species worldwide. This fungus forms melanized microsclerotia which help it to survive under adverse conditions and these structures are vital to the disease spread. Here, we identified and characterized a V. dahliae homolog to of the Saccharomyces cerevisiae Ssk1, a response regulator of the two-component system. Herein, we demonstrated that the VdSsk1 deletion strains were more sensitive to various stresses, including oxidative stress conferred by H
2 O2 and sodium nitroprusside dihydrate, while the mutants confered higher resistance to fungicides such as fludioxonil and iprodione. Furthermore, disruption of VdSsk1 resulted in significant downregulation of melanin biosynthesis-related genes but did not affect microsclerotial development. Phosphorylation of VdHog1 was not detected in the VdSsk1 deletion strains under the treatment of sorbitol, indicating that phosphorylation of VdHog1 is dependent on VdSsk1. Finally, we demonstrated that VdSsk1 is required for full virulence. Taken together, this study suggests that VdSsk1 modulates stress response, melanin biosynthesis and virulence of V. dahliae. [ABSTRACT FROM AUTHOR]- Published
- 2019
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- View/download PDF
12. The mitogen-activated protein kinase gene, VdHog1, regulates osmotic stress response, microsclerotia formation and virulence in Verticillium dahliae.
- Author
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Wang, Yonglin, Tian, Longyan, Xiong, Dianguang, Klosterman, Steven J., Xiao, Shuxiao, and Tian, Chengming
- Subjects
- *
MITOGEN-activated protein kinases , *OSMOTIC pressure , *MICROBIAL virulence , *VERTICILLIUM dahliae , *HYDROPHOBINS - Abstract
The fungus Verticillium dahliae has gained worldwide notoriety as a destructive plant pathogen, causing vascular wilt diseases on diverse plant species. V. dahliae produces melanized resting bodies, known as microsclerotia, which can survive for 15 years in the soil, and are thus critically important in its disease cycle. However, the molecular mechanisms that underpin microsclerotia formation, survival, and germination remain poorly understood. In this study, we observed that deletion of VdHog1 (Δ VdHog1 ), encoding a homolog of a high-osmolarity glycerol (HOG) response mitogen-activated protein kinase, displayed decreased numbers of melanized microsclerotia in culture, heightened sensitivity to hyperosmotic stress, and increased resistance to the fungicide fludioxonil. Through RNA-Seq analysis, we identified 221 genes differentially expressed in the Δ VdHog1 strain. Interestingly, the expression levels of genes involved in melanin biosynthesis, as well as the hydrophobin gene VDH1 , involved in the early stage of microsclerotia formation, were significantly decreased in the Δ VdHog1 strains relative to the wild-type expression levels. The Δ VdHog1 strains exhibited decreased virulence relative to the wild type strain on smoke tree seedlings. These results indicate that VdHog1 regulates hyperosmotic stress responses in V. dahliae , and establishes the Hog1-mediated pathway as a target to further probe the up- and downstream processes that regulate asexual development in this fungus. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
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13. VdCrz1 is involved in microsclerotia formation and required for full virulence in Verticillium dahliae.
- Author
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Xiong, Dianguang, Wang, Yonglin, Tang, Chen, Fang, Yulin, Zou, Jingyi, and Tian, Chengming
- Subjects
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VERTICILLIUM dahliae , *PATHOGENIC fungi , *CALCINEURIN , *PHYTOPATHOGENIC fungi , *TRANSCRIPTION factors - Abstract
Calcium signaling plays crucial roles in ion stress tolerance, sporulation and pathogenicity in fungi. Although the signaling pathway mediated by calcineurin and the calcineurin-responsive zinc finger transcription factor Crz1 is well characterized in other fungi, this pathway is not well characterized in the phytopathogenic fungus, Verticillium dahliae . To better understand the role of this calcineurin-dependent transcription factor in V. dahliae , an ortholog of CRZ1 , VdCrz1 , was identified and characterized functionally. Transcriptional analysis of VdCrz1 and GFP expression driven by the VdCrz1 promoter indicated that VdCrz1 was involved in microsclerotia development. After targeted deletion of VdCrz1 , microsclerotia formation and melanin accumulation were impaired. Furthermore, the Δ VdCrz1 mutants were hypersensitive to high concentrations of Ca 2+ and cell wall-perturbing agents, such as sodium dodecyl sulfate. The addition of Mg 2+ to the medium restores the microsclerotia formation in Δ VdCrz1 mutants. The Δ VdCrz1 mutants exhibited delayed Verticillium wilt symptoms on smoke tree. These results suggest that VdCrz1 plays important roles in Ca 2+ signaling, cell wall integrity, microsclerotia development and full virulence in V. dahliae . [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
14. Phylogenic analysis revealed an expanded C2H2-homeobox subfamily and expression profiles of C2H2 zinc finger gene family in Verticillium dahliae.
- Author
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Xiong, Dianguang, Wang, Yonglin, Deng, Chenglin, Hu, Ruowen, and Tian, Chengming
- Subjects
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HOMEOBOX genes , *GENE expression , *VERTICILLIUM dahliae , *PHYLOGENY , *ZINC-finger proteins , *FUSARIUM oxysporum - Abstract
C 2 H 2 zinc finger (CZF) proteins are a major class of transcription factors that play crucial roles in fungal growth, development, various stress responses, and virulence. Little genome-wide data is available regarding the roles of CZF proteins in Verticillium dahliae , a destructive pathogen that causes vascular wilt disease in more than 200 plant species. We identified a total of 79 typical CZF genes in V. dahliae . Comparative analysis revealed that four plant pathogenic fungi, V. dahliae , Fusarium oxysporum , Magnaporthe oryzae , and Botrytis cinerea , have comparable numbers of predicted CZF genes with similar characteristics. Phylogenetic analysis identified a C 2 H 2 -homeobox subfamily in V. dahliae containing seven genes with similar gene structures. V. dahliae and F. oxysporum (Hypocreomycetidae) have more genes of this subfamily than M. oryzae (Sordariomycetidae) and B. cinerea (Leotiomycetes). Furthermore, gene-expression analysis of the smoke tree wilt fungus V. dahliae strain XS11 using digital gene-expression profiling and RT-qPCR revealed that a number of CZF genes were differentially expressed during microsclerotia formation, nutritional starvation, and simulated in planta conditions. Furthermore, the expression profiles revealed that some CZF genes were overrepresented during multiple stages, indicating that they might play diverse roles. Our results provide useful information concerning the functions of CZF genes in microsclerotia formation, nutritional stress responses, and pathogenicity in V. dahliae , and form a basis for future functional studies of these genes. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
15. Genome-Wide Identification, Phylogeny and Expression Profile of Vesicle Fusion Components in Verticillium dahliae.
- Author
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Yang, Xue, Ben, Siqi, Sun, Yingjiao, Fan, Xinlei, Tian, Chengming, and Wang, Yonglin
- Subjects
PHYLOGENY ,GENE expression ,VERTICILLIUM dahliae ,MALEIMIDES ,SNARE proteins ,NUCLEOTIDE sequence ,VASCULAR diseases - Abstract
Vesicular trafficking plays a crucial role in protein localization and movement, signal transduction, and multiple developmental processes in eukaryotic cells. Vesicle fusion is the final and key step in vesicle-mediated trafficking and mainly relies on SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), the regulators including SM (Sec1/Munc18) family proteins, Rab GTPases and exocyst subunits. Verticillium dahliae is a widespread soil fungus that causes disruptive vascular diseases on a wide range of plants. To date, no genes involved in vesicular fusion process have been identified and characterized in V. dahliae. The recent publication of the draft genome sequence of V. dahliae allowed us to conduct a genome-wide identification, phylogeny and expression profile of genes encoding vesicular fusion components. Using compared genomics and phylogenetic methods, we identified 44 genes encoding vesicle fusion components in the V. dahliae genome. According to the structural features of their encoded proteins, the 44 V. dahliae genes were classified into 22 SNAREs (6 Qa-, 4 Qb-, 6 Qc-, 1 Qbc- and 5 R-types), 4 SM family proteins, 10 Rab GTPases and 8 exocyst proteins. Based on phylogeny and motif constitution analysis, orthologs of vesicle fusion component in filamentous fungi were generally clustered together into the same subclasses with well-supported bootstrap values. Analysis of the expression profiles of these genes indicated that many of them are significantly differentially expressed during vegetative growth and microsclerotia formation in V. dahliae. The analysis show that many components of vesicle fusion are well conserved in filamentous fungi and indicate that vesicle fusion plays a critical role in microsclerotia formation of smoke tree wilt fungus V. dahliae. The genome-wide identification and expression analysis of components involved in vesicle fusion should facilitate research in this gene family and give new insights toward elucidating their functions in growth, development and pathogenesis of V. dahliae. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
16. The C2H2 transcription factor VdMsn2 controls hyphal growth, microsclerotia formation, and virulence of Verticillium dahliae.
- Author
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Tian, Longyan, Yu, Jun, Wang, Yonglin, and Tian, Chengming
- Subjects
- *
VERTICILLIUM dahliae , *TRANSCRIPTION factors , *HYPHAE of fungi , *SCLEROTIUM (Mycelium) , *PHYTOPATHOGENIC fungi - Abstract
Verticillium dahliae is a notorious pathogen that causes vascular wilt disease in numerous plant species worldwide. The fungus produces melanized microsclerotia, which helps it survive adverse environmental conditions that it may encounter within its hosts and in the soil. Previously, we determined that the high osmolarity glycerol (HOG) pathway is involved in the environmental stress response of V. dahliae . In this study, we investigated the function of VdMsn2, a homologue of the yeast C 2 H 2 transcription factor Msn2, which is predicted to function as a downstream player in the HOG pathway. Disruption of VdMsn2 has a discernible effect on hyphal growth and septation, but not on diverse stresses including hyperosmotic stresses and cell wall inhibitory agents. Furthermore, we show that VdMsn2 deletion mutants produce significantly more microsclerotia than the wild-type and exhibit attenuated virulence to smoke trees because of poor penetration. Taken together, our findings suggest that VdMsn2 controls hyphal growth, microsclerotia formation, and virulence but does not significantly contribute to stress responses in V. dahliae . [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
17. Kss1 of Verticillium dahliae regulates virulence, microsclerotia formation, and nitrogen metabolism.
- Author
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Li, Wenwen, Li, Sa, Tang, Chen, Klosterman, Steven J., and Wang, Yonglin
- Subjects
- *
VERTICILLIUM dahliae , *WILT diseases , *PROTEIN kinases , *METABOLIC regulation , *ORNAMENTAL trees , *METABOLISM - Abstract
Verticillium dahliae causes destructive vascular wilt diseases on more than 200 plant species, including economically important crops and ornamental trees worldwide. The melanized microsclerotia (MS) enable V. dahliae to survive for years in soil, thus the fungus is especially difficult to control once it has become established. Previously, we found that the mitogen activated protein kinase VdSte11 (MAPKKK) plays key roles in MS formation, penetration, and virulence in V. dahliae. In this study, two MAPK homologs of the yeast Ste7p and Kss1p were identified and characterized in V. dahliae. Deletion of VdSte7 or VdKss1 reuslted in severe defects in melaninized MS formation and virulence. Furthermore, phosphorylation assays demonstrated that VdSte11 and VdSte7 can phosphorylate VdKss1 in V. dahliae. Proteomic analysis revealed a significant change in sterol biosynthesis with a fold change of ≥ 1.2 after the deletion of VdKss1. In addition, phosphoproteomic analysis showed that VdKss1 was involved in the regulation of nitrogen metabolism. Finally, we identified VdRlm1 as a potentially downstream target of VdKss1, which is involved in regulating ammonium nitrogen utilization. This study sheds light on the network of regulatory proteins in V. dahliae that affect MS formation and nitrogen metabolism. [Display omitted] • The VdKss1 MAPK signaling affects microsclerotia formation and virulence. • VdSte7-VdKss1 kinase module is important for penetration peg formation. • VdKss1 MAPK signaling regulates nitric oxide (NO) resistance and inorganic nitrogen metabolism. • VdRlm1 functions as a downstream target transcription factor phosphorylated by VdKss1, participating in the regulation of the utilization of preferred nitrogen sources. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
18. The two-component response regulator VdSkn7 plays key roles in microsclerotial development, stress resistance and virulence of Verticillium dahliae.
- Author
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Tang, Chen, Xiong, Dianguang, Fang, Yulin, Tian, Chengming, and Wang, Yonglin
- Subjects
- *
VERTICILLIUM dahliae , *SCLEROTIUM (Mycelium) , *ABIOTIC stress , *SACCHAROMYCES cerevisiae , *HOST-fungus relationships - Abstract
The fungus Verticillium dahliae causes vascular wilt disease on various plant species resulting in devastating yield losses worldwide. The capacity of V. dahliae to colonize in host plant xylem and disseminate by microsclerotia has led to studies to evaluate genes associated with pathogenesis and microsclerotia formation. Here, we identified and characterized a V. dahliae homolog to Skn7, a two-component stress response regulator of Saccharomyces cerevisiae . Results showed that melanized microsclerotia formation and conidiation were significantly inhibited in the VdSkn7 deletion mutants. VdSkn7 -deficient mutants displayed severe growth defect under heat shock, cell wall perturbing agents and H 2 O 2 , and were significantly less virulent but were not sensitive to osmotic stresses compared to the wild-type strain. Finally, we demonstrated that VdSkn7 is required for the plant penetration. Taken together, our study thus provides new evidence on the functional conservation and divergence of Skn7 orthologs among fungal organisms and indicates that VdSkn7 contributes to microsclerotial development, virulence and stress response of V. dahliae . [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
19. Functional characterization of two bZIP transcription factors in Verticillium dahliae.
- Author
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Fang, Yulin, Xiong, Dianguang, Tian, Longyan, Tang, Chen, Wang, Yonglin, and Tian, Chengming
- Subjects
- *
LEUCINE zippers , *VERTICILLIUM dahliae , *TRANSCRIPTION factors , *FUNGAL virulence , *OXIDATIVE stress , *PATHOGENIC fungi , *PHYSIOLOGY , *FUNGI - Abstract
bZIP transcription factors play various biological roles in stress responses, conidiation, and pathogenicity in pathogenic fungi. Here, we report two bZIP transcription factors (VDAG_08640 and VDAG_08676) of Verticillium dahliae , which were differentially expressed during microsclerotia development and induced by hydrogen peroxide as well. We find that deletion of either gene does not affect microsclerotia formation and the sensitivity to hydrogen peroxide; however, the mutants manifest decreased activity of extracellular peroxidase and laccase. Other phenotypic characterization reveals that VDAG_08676 disruption results in significant reduction of conidial production and virulence, while VDAG_08640 disruption does not lead to observable phenotypic variances compared with the wild-type strain. To elucidate whether they exhibit functional redundancy, double deletion mutants were generated. The double deletion mutants show remarkably increased sensitivity to hydrogen peroxide stress, whereas the two genes are not involved in microsclerotia formation. Taken together, our data demonstrate that a bZIP transcription factor gene VDAG_08676 is involved in the conidial production, oxidative stress response and virulence which may lay a foundation for further analysis of other bZIP transcription factors in V. dahliae . [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
20. Genome-Wide Identification, Phylogeny and Expression Profile of Vesicle Fusion Components in Verticillium dahliae.
- Author
-
Yang, Xue, Ben, Siqi, Sun, Yingjiao, Fan, Xinlei, Tian, Chengming, and Wang, Yonglin
- Subjects
- *
PHYLOGENY , *GENE expression , *VERTICILLIUM dahliae , *MALEIMIDES , *SNARE proteins , *NUCLEOTIDE sequence , *VASCULAR diseases - Abstract
Vesicular trafficking plays a crucial role in protein localization and movement, signal transduction, and multiple developmental processes in eukaryotic cells. Vesicle fusion is the final and key step in vesicle-mediated trafficking and mainly relies on SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), the regulators including SM (Sec1/Munc18) family proteins, Rab GTPases and exocyst subunits. Verticillium dahliae is a widespread soil fungus that causes disruptive vascular diseases on a wide range of plants. To date, no genes involved in vesicular fusion process have been identified and characterized in V. dahliae. The recent publication of the draft genome sequence of V. dahliae allowed us to conduct a genome-wide identification, phylogeny and expression profile of genes encoding vesicular fusion components. Using compared genomics and phylogenetic methods, we identified 44 genes encoding vesicle fusion components in the V. dahliae genome. According to the structural features of their encoded proteins, the 44 V. dahliae genes were classified into 22 SNAREs (6 Qa-, 4 Qb-, 6 Qc-, 1 Qbc- and 5 R-types), 4 SM family proteins, 10 Rab GTPases and 8 exocyst proteins. Based on phylogeny and motif constitution analysis, orthologs of vesicle fusion component in filamentous fungi were generally clustered together into the same subclasses with well-supported bootstrap values. Analysis of the expression profiles of these genes indicated that many of them are significantly differentially expressed during vegetative growth and microsclerotia formation in V. dahliae. The analysis show that many components of vesicle fusion are well conserved in filamentous fungi and indicate that vesicle fusion plays a critical role in microsclerotia formation of smoke tree wilt fungus V. dahliae. The genome-wide identification and expression analysis of components involved in vesicle fusion should facilitate research in this gene family and give new insights toward elucidating their functions in growth, development and pathogenesis of V. dahliae. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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