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Pineapple is a plant whose fruit has economic value, so it needs to be developed on a plantation scale. This study aims to determine the best combination of media for the growth of in vitro rooting Sipahutar pineapple. Complete Random Design was used with 12 treatments with 3 replications in this study. The parameters used in the observation were divided into the number of shoots, number of leaves and number of roots. From the results of the data analyzed, it can be seen that in the treatment of MS+2 ppm Vitamin+2 ppm Naphthalene Acetic Acid (NAA) media, the highest number of shoots was obtained, namely at 18.66 shoots on 12 week after planting (WAP) observations. The lowest number of shoots on leaves was 11.33, the results of data analysis showed that the highest number of leaf shoots was 8.00 on 12 WAP observations on MS+2 ppmVitamin+2 ppm NAA media treatment. From the treatment of MS media+1 ppm Vitamin+1 ppm NAA and MS+2 ppm Vitamin+0 ppm NAA, the lowest number of leaf shoots was obtained with an average of 0.33, while the results of data analysis on root propagation resulted in the highest number of leaf shoots 8.00 in.12 WAP observations on MS media+2 ppm Vitamin treatment.+2 ppm NAA. [ABSTRACT FROM AUTHOR]