1. Microfluidic-prepared DOTAP nanoparticles induce strong T-cell responses in mice.
- Author
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Haseda Y, Munakata L, Meng J, Suzuki R, and Aoshi T
- Subjects
- Adjuvants, Immunologic chemistry, Animals, CD4-Positive T-Lymphocytes drug effects, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes drug effects, CD8-Positive T-Lymphocytes immunology, Humans, Immunity, Cellular immunology, Liposomes pharmacology, Mice, Microfluidics, Nanoparticles chemistry, Vaccines chemistry, Adjuvants, Immunologic pharmacology, Fatty Acids, Monounsaturated pharmacology, Immunity, Cellular drug effects, Quaternary Ammonium Compounds pharmacology, Vaccines pharmacology
- Abstract
For the induction of antigen-specific T-cell responses by vaccination, an appropriate immune adjuvant is required. Vaccine adjuvants generally provide two functions, namely, immune potentiator and delivery, and many adjuvants that can efficiently induce T-cell responses are known to have the combination of these two functions. In this study, we explored a cationic lipid DOTAP-based adjuvant. We found that the microfluidic preparation of DOTAP nanoparticles induced stronger CD4+ and CD8+ T-cell responses than liposomal DOTAP. The further addition of Type-A CpG D35 in DOTAP nanoparticles increased the induction of T-cell responses, particularly in CD4+ T cells. Further investigations revealed that the size of DOTAP nanoparticles, prepared buffer conditions, and physicochemical interaction with vaccine antigen are important factors for the efficient induction of T-cell responses with a relatively small antigen dose. These results suggested that microfluidic-prepared DOTAP nanoparticles plus D35 are a promising adjuvant for a vaccine that induces therapeutic T-cell responses for treating cancer and infectious diseases., Competing Interests: Some of the authors have filed a patent application (Application Number 2018-227061) related to the content of this manuscript. However, this does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2020
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